Ethanol-induced Oxidative Stress: Basic Knowledge

Overview

Journal Genes Nutr

Publisher Biomed Central

Date 2010 Jul 8

PMID 20606811

Citations 52

Authors

Mario Comporti

Cinzia Signorini

Silvia Leoncini

Concetta Gardi

Lucia Ciccoli

Anna Giardini

Daniela Vecchio

Beatrice Arezzini

Affiliations

Soon will be listed here.

Abstract

After a general introduction, the main pathways of ethanol metabolism (alcohol dehydrogenase, catalase, coupling of catalase with NADPH oxidase and microsomal ethanol-oxidizing system) are shortly reviewed. The cytochrome P(450) isoform (CYP2E1) specifically involved in ethanol oxidation is discussed. The acetaldehyde metabolism and the shift of the NAD/NADH ratio in the cellular environment (reductive stress) are stressed. The toxic effects of acetaldehyde are mentioned. The ethanol-induced oxidative stress: the increased MDA formation by incubated liver preparations, the absorption of conjugated dienes in mitochondrial and microsomal lipids and the decrease in the most unsaturated fatty acids in liver cell membranes are discussed. The formation of carbon-centered (1-hydroxyethyl) and oxygen-centered (hydroxyl) radicals during the metabolism of ethanol is considered: the generation of hydroxyethyl radicals, which occurs likely during the process of univalent reduction of dioxygen, is highlighted and is carried out by ferric cytochrome P(450) oxy-complex (P(450)-Fe(3+)O(2) (.-)) formed during the reduction of heme-oxygen. The ethanol-induced lipid peroxidation has been evaluated, and it has been shown that plasma F(2)-isoprostanes are increased in ethanol toxicity.

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References

Aleynik S, Leo M, Aleynik M, Lieber C . Increased circulating products of lipid peroxidation in patients with alcoholic liver disease. Alcohol Clin Exp Res. 1998; 22(1):192-6. View

Hildebrandt A, Speck M, Roots I . Possible control of hydrogen peroxide production and degradation in microsomes during mixed function oxidation reaction. Biochem Biophys Res Commun. 1973; 54(3):968-75. DOI: 10.1016/0006-291x(73)90789-4. View

LANE B, Lieber C . Ultrastructural alterations in human hepatocytes following ingestion of ethanol with adequate diets. Am J Pathol. 1966; 49(4):593-603. PMC: 1907245. View

KREBS H, Perkins J . The physiological role of liver alcohol dehydrogenase. Biochem J. 1970; 118(4):635-44. PMC: 1179260. DOI: 10.1042/bj1180635. View

Pemberton P, Smith A, Warnes T . Non-invasive monitoring of oxidant stress in alcoholic liver disease. Scand J Gastroenterol. 2005; 40(9):1102-8. DOI: 10.1080/00365520510023495. View

Lieber C, DECARLI L . The role of the hepatic microsomal ethanol oxidizing system (MEOS) for ethanol metabolism in vivo. J Pharmacol Exp Ther. 1972; 181(2):279-87. View

DIANZANI M, Baccino F, Comporti M . The direct effect of carbon tetrachloride on subcellular particles. Lab Invest. 1966; 15(1 Pt 1):149-56. View

Comporti M, Burdino E, Raja F . Fatty acid composition of mitochondrial and microsomal lipids of rat liver after acute ethanol intoxication. Life Sci II. 1971; 10(15):855-66. DOI: 10.1016/0024-3205(71)90198-6. View

Lieber C, DECARLI L . Ethanol oxidation by hepatic microsomes: adaptive increase after ethanol feeding. Science. 1968; 162(3856):917-8. DOI: 10.1126/science.162.3856.917. View

10.

Ontko J . Effects of ethanol on the metabolism of free fatty acids in isolated liver cells. J Lipid Res. 1973; 14(1):78-86. View

11.

MONCADA C, Torres V, Varghese G, Albano E, Israel Y . Ethanol-derived immunoreactive species formed by free radical mechanisms. Mol Pharmacol. 1994; 46(4):786-91. View

12.

Schroder M . Endoplasmic reticulum stress responses. Cell Mol Life Sci. 2007; 65(6):862-94. PMC: 11131897. DOI: 10.1007/s00018-007-7383-5. View

13.

DiLUZIO N . PREVENTION OF THE ACUTE ETHANOL-INDUCED FATTY LIVER BY THE SIMULTANEOUS ADMINISTRATION OF ANTIOXIDANTS. Life Sci (1962). 1964; 3:113-8. DOI: 10.1016/0024-3205(64)90189-4. View

14.

Albano E, Tomasi A, Persson J, Terelius Y, Ingelman-Sundberg M, DIANZANI M . Role of ethanol-inducible cytochrome P450 (P450IIE1) in catalysing the free radical activation of aliphatic alcohols. Biochem Pharmacol. 1991; 41(12):1895-902. DOI: 10.1016/0006-2952(91)90129-s. View

15.

Baudhuin P . Liver peroxisomes, cytology and function. Ann N Y Acad Sci. 1969; 168(2):214-28. DOI: 10.1111/j.1749-6632.1969.tb43111.x. View

16.

Cederbaum A, Rubin E . Molecular injury to mitochondria produced by ethanol and acetaldehyde. Fed Proc. 1975; 34(11):2045-51. View

17.

McManus I, Contag A, Olson R . Characterization of Endogenous Ethanol in the Mammal. Science. 1960; 131(3393):102-3. DOI: 10.1126/science.131.3393.102. View

18.

Reinke L, Kotake Y, MCCAY P, Janzen E . Spin-trapping studies of hepatic free radicals formed following the acute administration of ethanol to rats: in vivo detection of 1-hydroxyethyl radicals with PBN. Free Radic Biol Med. 1991; 11(1):31-9. DOI: 10.1016/0891-5849(91)90185-6. View

19.

BEST C, HARTROFT W . Liver damage produced by feeding alcohol or sugar and its prevention by choline. Br Med J. 1949; 2(4635):1002-6, pl. PMC: 2051633. DOI: 10.1136/bmj.2.4635.1001. View

20.

Rubin E, Lieber C . Hepatic microsomal enzymes in man and rat: induction and inhibition by ethanol. Science. 1968; 162(3854):690-1. DOI: 10.1126/science.162.3854.690. View