Understanding Lipid Rafts and Other Related Membrane Domains

Overview

Journal F1000 Biol Rep

Specialty Biology

Date 2010 Jul 8

PMID 20606718

Citations 15

Authors

Aaron K Neumann

Michelle S Itano

Ken Jacobson

Affiliations

Soon will be listed here.

Abstract

Evidence in support of the classical lipid raft hypothesis has remained elusive. Data suggests that transmembrane proteins and the actin-containing cortical cytoskeleton can organize lipids into short-lived nanoscale assemblies that can be assembled into larger domains under certain conditions. This supports an evolving view in which interactions between lipids, cholesterol, and proteins create and maintain lateral heterogeneity in the cell membrane.

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References

Chen Y, Thelin W, Yang B, Milgram S, Jacobson K . Transient anchorage of cross-linked glycosyl-phosphatidylinositol-anchored proteins depends on cholesterol, Src family kinases, caveolin, and phosphoinositides. J Cell Biol. 2006; 175(1):169-78. PMC: 2064508. DOI: 10.1083/jcb.200512116. View

Saad J, Miller J, Tai J, Kim A, Ghanam R, Summers M . Structural basis for targeting HIV-1 Gag proteins to the plasma membrane for virus assembly. Proc Natl Acad Sci U S A. 2006; 103(30):11364-9. PMC: 1544092. DOI: 10.1073/pnas.0602818103. View

Glebov O, Nichols B . Lipid raft proteins have a random distribution during localized activation of the T-cell receptor. Nat Cell Biol. 2004; 6(3):238-43. DOI: 10.1038/ncb1103. View

Aloia R, Tian H, JENSEN F . Lipid composition and fluidity of the human immunodeficiency virus envelope and host cell plasma membranes. Proc Natl Acad Sci U S A. 1993; 90(11):5181-5. PMC: 46679. DOI: 10.1073/pnas.90.11.5181. View

Lenne P, Wawrezinieck L, Conchonaud F, Wurtz O, Boned A, Guo X . Dynamic molecular confinement in the plasma membrane by microdomains and the cytoskeleton meshwork. EMBO J. 2006; 25(14):3245-56. PMC: 1523176. DOI: 10.1038/sj.emboj.7601214. View

Engel S, Scolari S, Thaa B, Krebs N, Korte T, Herrmann A . FLIM-FRET and FRAP reveal association of influenza virus haemagglutinin with membrane rafts. Biochem J. 2009; 425(3):567-73. DOI: 10.1042/BJ20091388. View

Kalvodova L, Sampaio J, Cordo S, Ejsing C, Shevchenko A, Simons K . The lipidomes of vesicular stomatitis virus, semliki forest virus, and the host plasma membrane analyzed by quantitative shotgun mass spectrometry. J Virol. 2009; 83(16):7996-8003. PMC: 2715782. DOI: 10.1128/JVI.00635-09. View

van Meer G, Simons K . Viruses budding from either the apical or the basolateral plasma membrane domain of MDCK cells have unique phospholipid compositions. EMBO J. 1982; 1(7):847-52. PMC: 553120. DOI: 10.1002/j.1460-2075.1982.tb01258.x. View

Jouvenet N, Bieniasz P, Simon S . Imaging the biogenesis of individual HIV-1 virions in live cells. Nature. 2008; 454(7201):236-40. PMC: 2708942. DOI: 10.1038/nature06998. View

10.

Ivanchenko S, Godinez W, Lampe M, Krausslich H, Eils R, Rohr K . Dynamics of HIV-1 assembly and release. PLoS Pathog. 2009; 5(11):e1000652. PMC: 2766258. DOI: 10.1371/journal.ppat.1000652. View

11.

Jolly C, Sattentau Q . Human immunodeficiency virus type 1 assembly, budding, and cell-cell spread in T cells take place in tetraspanin-enriched plasma membrane domains. J Virol. 2007; 81(15):7873-84. PMC: 1951303. DOI: 10.1128/JVI.01845-06. View

12.

Goswami D, Gowrishankar K, Bilgrami S, Ghosh S, Raghupathy R, Chadda R . Nanoclusters of GPI-anchored proteins are formed by cortical actin-driven activity. Cell. 2008; 135(6):1085-97. PMC: 7616455. DOI: 10.1016/j.cell.2008.11.032. View

13.

Sengupta P, Hammond A, Holowka D, Baird B . Structural determinants for partitioning of lipids and proteins between coexisting fluid phases in giant plasma membrane vesicles. Biochim Biophys Acta. 2007; 1778(1):20-32. PMC: 2679899. DOI: 10.1016/j.bbamem.2007.08.028. View

14.

Kenworthy A, Nichols B, Remmert C, Hendrix G, Kumar M, Zimmerberg J . Dynamics of putative raft-associated proteins at the cell surface. J Cell Biol. 2004; 165(5):735-46. PMC: 2172371. DOI: 10.1083/jcb.200312170. View

15.

Grigorian A, Torossian S, Demetriou M . T-cell growth, cell surface organization, and the galectin-glycoprotein lattice. Immunol Rev. 2009; 230(1):232-46. PMC: 3059806. DOI: 10.1111/j.1600-065X.2009.00796.x. View

16.

Hemler M . Tetraspanin functions and associated microdomains. Nat Rev Mol Cell Biol. 2005; 6(10):801-11. DOI: 10.1038/nrm1736. View

17.

Koopman M, Cambi A, de Bakker B, Joosten B, Figdor C, van Hulst N . Near-field scanning optical microscopy in liquid for high resolution single molecule detection on dendritic cells. FEBS Lett. 2004; 573(1-3):6-10. DOI: 10.1016/j.febslet.2004.07.035. View

18.

Cambi A, de Lange F, van Maarseveen N, Nijhuis M, Joosten B, van Dijk E . Microdomains of the C-type lectin DC-SIGN are portals for virus entry into dendritic cells. J Cell Biol. 2004; 164(1):145-55. PMC: 2171967. DOI: 10.1083/jcb.200306112. View

19.

Dietrich C, Bagatolli L, Volovyk Z, Thompson N, Levi M, Jacobson K . Lipid rafts reconstituted in model membranes. Biophys J. 2001; 80(3):1417-28. PMC: 1301333. DOI: 10.1016/S0006-3495(01)76114-0. View

20.

Polozov I, Bezrukov L, Gawrisch K, Zimmerberg J . Progressive ordering with decreasing temperature of the phospholipids of influenza virus. Nat Chem Biol. 2008; 4(4):248-55. DOI: 10.1038/nchembio.77. View