Insecticidal Activity of Two Proteases Against Spodoptera Frugiperda Larvae Infected with Recombinant Baculoviruses

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2010 Jul 1

PMID 20587066

Citations 11

Authors

Aline Welzel Gramkow

Simone Perecmanis

Raul Lima Barbosa Sousa

Eliane Ferreira Noronha

Carlos Roberto Felix

Tatsuya Nagata

Bergmann Morais Ribeiro

Affiliations

Soon will be listed here.

Abstract

Background: Baculovirus comprise the largest group of insect viruses most studied worldwide, mainly because they efficiently kill agricultural insect pests. In this study, two recombinant baculoviruses containing the ScathL gene from Sarcophaga peregrina (vSynScathL), and the Keratinase gene from the fungus Aspergillus fumigatus (vSynKerat), were constructed, and their insecticidal properties analysed against Spodoptera frugiperda larvae.

Results: Bioassays of third-instar and neonate S. frugiperda larvae with vSynScathL and vSynKerat showed a decrease in the time needed to kill the infected insects when compared to the wild type virus. We have also shown that both recombinants were able to increase phenoloxidase activity in the hemolymph of S. frugiperda larvae. The expression of proteases in infected larvae resulted in destruction of internal tissues late in infection, which could be the reason for the increased viral speed of kill.

Conclusions: Baculoviruses and their recombinant forms constitute viable alternatives to chemical insecticides. Recombinant baculoviruses containing protease genes can be added to the list of engineered baculoviruses with great potential to be used in integrated pest management programs.

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