Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2010 Jun 30

PMID 20585575

Citations 73

Authors

Irene Cervello

Claudia Gil-Sanchis

Aymara Mas

Francisco Delgado-Rosas

Jose Antonio Martinez-Conejero

Amparo Galan

Alicia Martinez-Romero

Sebastian Martinez

Ismael Navarro

Jaime Ferro

Jose Antonio Horcajadas

Francisco Jose Esteban

Jose Enrique OConnor

Antonio Pellicer

Carlos Simon

Affiliations

Soon will be listed here.

Abstract

During reproductive life, the human endometrium undergoes around 480 cycles of growth, breakdown and regeneration should pregnancy not be achieved. This outstanding regenerative capacity is the basis for women's cycling and its dysfunction may be involved in the etiology of pathological disorders. Therefore, the human endometrial tissue must rely on a remarkable endometrial somatic stem cells (SSC) population. Here we explore the hypothesis that human endometrial side population (SP) cells correspond to somatic stem cells. We isolated, identified and characterized the SP corresponding to the stromal and epithelial compartments using endometrial SP genes signature, immunophenotyping and characteristic telomerase pattern. We analyzed the clonogenic activity of SP cells under hypoxic conditions and the differentiation capacity in vitro to adipogenic and osteogenic lineages. Finally, we demonstrated the functional capability of endometrial SP to develop human endometrium after subcutaneous injection in NOD-SCID mice. Briefly, SP cells of human endometrium from epithelial and stromal compartments display genotypic, phenotypic and functional features of SSC.

Citing Articles

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