Data Analysis in Flow Cytometry: the Future Just Started

Overview

Journal Cytometry A

Specialties Cell Biology
Radiology

Date 2010 Jun 29

PMID 20583274

Citations 77

Authors

Enrico Lugli

Mario Roederer

Andrea Cossarizza

Affiliations

Soon will be listed here.

Abstract

In the last 10 years, a tremendous progress characterized flow cytometry in its different aspects. In particular, major advances have been conducted regarding the hardware/instrumentation and reagent development, thus allowing fine cell analysis up to 20 parameters. As a result, this technology generates very complex datasets that demand for the development of optimal tools of analysis. Recently, many independent research groups approached the problem by using both supervised and unsupervised methods. In this article, we will review the new developments concerning the use of bioinformatics for polychromatic flow cytometry and propose what should be done to unravel the enormous heterogeneity of the cells we interrogate each day.

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