HnRNP Q and PTB Modulate the Circadian Oscillation of Mouse Rev-erb Alpha Via IRES-mediated Translation

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2010 Jun 26

PMID 20576698

Citations 40

Authors

Do-Yeon Kim

Kyung-Chul Woo

Kyung-Ha Lee

Tae-Don Kim

Kyong-Tai Kim

Affiliations

Soon will be listed here.

Abstract

The physiological and behavioral circadian rhythms of most creatures are controlled by a harmony of functional relationships between clock genes. In mammals, several core clock genes show rhythmic profiles of their mRNA and protein expression. Among them, Rev-erb α functions as a transcriptional repressor, affecting expression patterns of other clock genes. For the continuous and robust oscillation of the molecular clock system, the levels of Rev-erb α protein are expected to be tightly regulated with the correct timing. Here, we demonstrate that Rev-erb α has an internal ribosomal entry site (IRES) in its 5' untranslated region. Furthermore, we demonstrate that heterogeneous nuclear ribonucleoprotein Q and polypyrimidine tract-binding protein (PTB) modulate the IRES-mediated translation of Rev-erb α. We suggest that the rhythmic binding affinity of hnRNP Q to the Rev-erb α IRES and the change in PTB cytosolic levels lead to maintenance of the oscillation profile of the Rev-erb α protein.

Citing Articles

SYNCRIP promotes cell cycle progression and lung tumorigenesis by modulating AURKB translation.

Kim H, Ryu H, Kang M, Lee N, Kim H, Lee D Cancer Commun (Lond). 2024; 45(2):138-142.

PMID: 39639599 PMC: 11833668. DOI: 10.1002/cac2.12634.

Circadian regulation of translation.

Lyu J, Zhuang Y, Lin Y RNA Biol. 2024; 21(1):14-24.

PMID: 39324589 PMC: 11441039. DOI: 10.1080/15476286.2024.2408524.

Circadian ribosome profiling reveals a role for the upstream open reading frame in sleep.

Millius A, Yamada R, Fujishima H, Maeda K, Standley D, Sumiyama K Proc Natl Acad Sci U S A. 2023; 120(40):e2214636120.

PMID: 37769257 PMC: 10556633. DOI: 10.1073/pnas.2214636120.

A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.

Qi X, Yan Q, Shang Y, Zhao R, Ding X, Gao S Cell Death Differ. 2022; 29(11):2233-2246.

PMID: 35538151 PMC: 9613757. DOI: 10.1038/s41418-022-01011-1.

CNOT1 regulates circadian behaviour through mRNA decay in a deadenylation-dependent manner.

Mohamed H, Takahashi A, Nishijima S, Adachi S, Murai I, Okamura H RNA Biol. 2022; 19(1):703-718.

PMID: 35510877 PMC: 9090297. DOI: 10.1080/15476286.2022.2071026.

References

Shah O, Anthony J, Kimball S, Jefferson L . 4E-BP1 and S6K1: translational integration sites for nutritional and hormonal information in muscle. Am J Physiol Endocrinol Metab. 2000; 279(4):E715-29. DOI: 10.1152/ajpendo.2000.279.4.E715. View

Kim T, Woo K, Cho S, Ha D, Jang S, Kim K . Rhythmic control of AANAT translation by hnRNP Q in circadian melatonin production. Genes Dev. 2007; 21(7):797-810. PMC: 1838531. DOI: 10.1101/gad.1519507. View

Shearman L, Sriram S, Weaver D, Maywood E, Chaves I, Zheng B . Interacting molecular loops in the mammalian circadian clock. Science. 2000; 288(5468):1013-9. DOI: 10.1126/science.288.5468.1013. View

Kondratov R, Chernov M, Kondratova A, Gorbacheva V, Gudkov A, Antoch M . BMAL1-dependent circadian oscillation of nuclear CLOCK: posttranslational events induced by dimerization of transcriptional activators of the mammalian clock system. Genes Dev. 2003; 17(15):1921-32. PMC: 196247. DOI: 10.1101/gad.1099503. View

Ganguly S, Coon S, Klein D . Control of melatonin synthesis in the mammalian pineal gland: the critical role of serotonin acetylation. Cell Tissue Res. 2002; 309(1):127-37. DOI: 10.1007/s00441-002-0579-y. View

Hahm B, Cho O, Kim J, Kim Y, Kim J, Oh Y . Polypyrimidine tract-binding protein interacts with HnRNP L. FEBS Lett. 1998; 425(3):401-6. DOI: 10.1016/s0014-5793(98)00269-5. View

Panda S, Hogenesch J, Kay S . Circadian rhythms from flies to human. Nature. 2002; 417(6886):329-35. DOI: 10.1038/417329a. View

Ramakrishnan S, Muscat G . The orphan Rev-erb nuclear receptors: a link between metabolism, circadian rhythm and inflammation?. Nucl Recept Signal. 2006; 4:e009. PMC: 1472670. DOI: 10.1621/nrs.04009. View

Mirsky H, Liu A, Welsh D, Kay S, Doyle 3rd F . A model of the cell-autonomous mammalian circadian clock. Proc Natl Acad Sci U S A. 2009; 106(27):11107-12. PMC: 2699375. DOI: 10.1073/pnas.0904837106. View

10.

Yu W, Zheng H, Houl J, Dauwalder B, Hardin P . PER-dependent rhythms in CLK phosphorylation and E-box binding regulate circadian transcription. Genes Dev. 2006; 20(6):723-33. PMC: 1434787. DOI: 10.1101/gad.1404406. View

11.

Florez J, Takahashi J . The circadian clock: from molecules to behaviour. Ann Med. 1995; 27(4):481-90. DOI: 10.3109/07853899709002457. View

12.

Pelletier J, Sonenberg N . Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Nature. 1988; 334(6180):320-5. DOI: 10.1038/334320a0. View

13.

Coste H, Rodriguez J . Orphan nuclear hormone receptor Rev-erbalpha regulates the human apolipoprotein CIII promoter. J Biol Chem. 2002; 277(30):27120-9. DOI: 10.1074/jbc.M203421200. View

14.

Chomez P, Neveu I, Mansen A, Kiesler E, Larsson L, Vennstrom B . Increased cell death and delayed development in the cerebellum of mice lacking the rev-erbA(alpha) orphan receptor. Development. 2000; 127(7):1489-98. DOI: 10.1242/dev.127.7.1489. View

15.

Schepens B, Tinton S, Bruynooghe Y, Parthoens E, Haegman M, Beyaert R . A role for hnRNP C1/C2 and Unr in internal initiation of translation during mitosis. EMBO J. 2006; 26(1):158-69. PMC: 1782369. DOI: 10.1038/sj.emboj.7601468. View

16.

Bushell M, Stoneley M, Kong Y, Hamilton T, Spriggs K, Dobbyn H . Polypyrimidine tract binding protein regulates IRES-mediated gene expression during apoptosis. Mol Cell. 2006; 23(3):401-12. DOI: 10.1016/j.molcel.2006.06.012. View

17.

Kafasla P, Morgner N, Poyry T, Curry S, Robinson C, Jackson R . Polypyrimidine tract binding protein stabilizes the encephalomyocarditis virus IRES structure via binding multiple sites in a unique orientation. Mol Cell. 2009; 34(5):556-68. DOI: 10.1016/j.molcel.2009.04.015. View

18.

Preitner N, Damiola F, Lopez-Molina L, Zakany J, Duboule D, Albrecht U . The orphan nuclear receptor REV-ERBalpha controls circadian transcription within the positive limb of the mammalian circadian oscillator. Cell. 2002; 110(2):251-60. DOI: 10.1016/s0092-8674(02)00825-5. View

19.

Balsalobre A, Damiola F, Schibler U . A serum shock induces circadian gene expression in mammalian tissue culture cells. Cell. 1998; 93(6):929-37. DOI: 10.1016/s0092-8674(00)81199-x. View

20.

Dobbyn H, Hill K, Hamilton T, Spriggs K, Pickering B, Coldwell M . Regulation of BAG-1 IRES-mediated translation following chemotoxic stress. Oncogene. 2007; 27(8):1167-74. PMC: 2570717. DOI: 10.1038/sj.onc.1210723. View