» Articles » PMID: 20569467

Characterization of a Diverse Secretome Generated by the Mouse Preimplantation Embryo in Vitro

Overview
Publisher Biomed Central
Date 2010 Jun 24
PMID 20569467
Citations 5
Authors
Affiliations
Soon will be listed here.
Abstract

This study investigates the suitability of surface-enhanced laser desorption and ionization time-of-flight (SELDI-TOF) and electrospray ionization (ESI) mass spectrometry for analysis of the proteins released by the mouse preimplantation embryo in vitro. SELDI-TOF analysis with CM10 or IMAC30 (but not Q10) protein chips detected a protein peak at m/z approximately 8570 released by both C57BL6 and hybrid embryos. No other peaks unique to the presence of the embryo were identified with this method. ESI mass spectrometry of tryptic digests of embryo-conditioned media identified a total of 20 proteins released during development from the zygote to blastocyst stage. Four proteins were expressed in at least 7 out of 8 cultures tested, one of these (lactate dehydrogenase B) was in all cultures. A further five proteins were in at least half of the cultures and 11 more proteins were in at least one culture. The expression of two of these proteins is essential for preimplantation embryo development (NLR family, pyrin domain containing 5 and peptidyl arginine deiminase, type VI). A further four proteins detected have roles in redox regulation of cells, and three others are capable of inducing post-translational modifications of proteins. This study shows the feasibility of ESI mass spectrometry for identifying the proteins secreted by the preimplantation embryo in vitro. This analysis identifies a range of targets that now require detailed functional analysis to assess whether their release by the embryo is an important property of early embryo development.

Citing Articles

The embryo non-invasive pre-implantation diagnosis era: how far are we?.

Del Collado M, Andrade G, Goncalves N, Fortini S, Perecin F, Carriero M Anim Reprod. 2023; 20(2):e20230069.

PMID: 37720726 PMC: 10503888. DOI: 10.1590/1984-3143-AR2023-0069.


Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro.

Raes A, Wydooghe E, Pavani K, Pascottini O, Van Steendam K, Dhaenens M Int J Mol Sci. 2023; 24(7).

PMID: 37047535 PMC: 10094850. DOI: 10.3390/ijms24076563.


Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success.

Braga D, Setti A, Lo Turco E, Cordeiro F, Cabral E, Cortezzi S J Assist Reprod Genet. 2016; 33(12):1571-1583.

PMID: 27614633 PMC: 5171892. DOI: 10.1007/s10815-016-0800-7.


Characterization of secreted proteins of 2-cell mouse embryos cultured in vitro to the blastocyst stage with and without protein supplementation.

Burch T, Yu L, Nyalwidhe J, Horcajadas J, Bocca S, Swanson R J Assist Reprod Genet. 2014; 31(6):757-65.

PMID: 24658922 PMC: 4048375. DOI: 10.1007/s10815-014-0207-2.


Secretome profile of mouse oocytes after activation using mass spectrum.

Peng Q, Yang H, Xue S, Shi L, Yu Q, Kuang Y J Assist Reprod Genet. 2012; 29(8):765-71.

PMID: 22573034 PMC: 3430788. DOI: 10.1007/s10815-012-9789-8.

References
1.
Ammit A, ONeill C . Studies of the nature of the binding by albumin of platelet-activating factor released from cells. J Biol Chem. 1997; 272(30):18772-8. DOI: 10.1074/jbc.272.30.18772. View

2.
Lane M, Gardner D . Lactate regulates pyruvate uptake and metabolism in the preimplantation mouse embryo. Biol Reprod. 1999; 62(1):16-22. DOI: 10.1095/biolreprod62.1.16. View

3.
ONeill C . Evidence for the requirement of autocrine growth factors for development of mouse preimplantation embryos in vitro. Biol Reprod. 1997; 56(1):229-37. DOI: 10.1095/biolreprod56.1.229. View

4.
Balakier H, Dziak E, Sojecki A, Librach C, Michalak M, Opas M . Calcium-binding proteins and calcium-release channels in human maturing oocytes, pronuclear zygotes and early preimplantation embryos. Hum Reprod. 2002; 17(11):2938-47. DOI: 10.1093/humrep/17.11.2938. View

5.
Warner C, Panda P, Almquist C, Xu Y . Preferential survival of mice expressing the Qa-2 antigen. J Reprod Fertil. 1993; 99(1):145-7. DOI: 10.1530/jrf.0.0990145. View