Integrated Viral DNA Sequences in Epstein-Barr Virus-converted Human Lymphoma Lines

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1978 Mar 1

PMID 205668

Citations 23

Authors

T Lindahl

Affiliations

Soon will be listed here.

Abstract

Most human lymphoid cell lines contain multiple copies of circular, nonintegrated Epstein-Barr virus (EBV) DNA molecules as well as viral DNA sequences with properties of integrated DNA. The physical state of the EBV DNA in a human lymphoma line that only contains one virus genome equivalent per cell has now been studied by three different methods, neutral CsCl density gradient centrifugation, actinomycin D-CsCl gradient centrifugation, and Hirt fractionation. This cell line, AW-Ramos, has been obtained by EBV infection in vitro of the apparently EBV-negative Ramos lymphoma line. The results indicate that the EBV DNA in AW-Ramos is present exclusively in a linearly integrated form. Similar data were obtained with two other EBV-converted sublines of Ramos cells.

Citing Articles

My journey to DNA repair.

Lindahl T Genomics Proteomics Bioinformatics. 2013; 11(1):2-7.

PMID: 23453014 PMC: 4357663. DOI: 10.1016/j.gpb.2012.12.001.

Herpesviruses and chromosomal integration.

Morissette G, Flamand L J Virol. 2010; 84(23):12100-9.

PMID: 20844040 PMC: 2976420. DOI: 10.1128/JVI.01169-10.

A shuttle system for transfer of YACs between yeast and mammalian cells.

Simpson K, Huxley C Nucleic Acids Res. 1996; 24(23):4693-9.

PMID: 8972855 PMC: 146308. DOI: 10.1093/nar/24.23.4693.

The search for a gene involved in the determination of limited duplicative capacity in human cells.

Calef E, Scotto dAbusco A, Bianchi E, GARGANO S, Fruscalzo A Genetica. 1994; 94(2-3):249-53.

PMID: 7896143 DOI: 10.1007/BF01443438.

Possible DNA-RNA tumor virus interaction in human lymphomas: expression of retroviral proteins in Ramos lymphoma lines is enhanced after conversion with Epstein-Barr virus.

Lasky R, Troy F Proc Natl Acad Sci U S A. 1984; 81(1):33-7.

PMID: 6320170 PMC: 344604. DOI: 10.1073/pnas.81.1.33.

References

Tomizawa J, ANRAKU N . MOLECULAR MECHANISMS OF GENETIC RECOMBINATION IN BACTERIOPHAGE. IV. ABSENCE OF POLYNUCLEOTIDE INTERRUPTION IN DNA OF T4 AND LAMBDA PHAGE PARTICLES, WITH SPECIAL REFERENCE TO HETEROZYGOSIS. J Mol Biol. 1965; 11:509-27. DOI: 10.1016/s0022-2836(65)80007-9. View

TOMIZAWA J, ANRAKU N . MOLECULAR MECHANISMS OF GENETIC RECOMBINATION IN BACTERIOPHAGE. II. JOINING OF PARENTAL DNA MOLECULES OF PHAGE T4. J Mol Biol. 1964; 8:516-40. DOI: 10.1016/s0022-2836(64)80009-7. View

Falk L, Bjursell G, Adams A, Lindahl T . Human lymphoblastoid cell lines derived from individuals without lymphoproliferative disease contain the same latent forms of Epstein-Barr virus DNA as those found in tumor cells. Int J Cancer. 1977; 20(2):173-80. DOI: 10.1002/ijc.2910200203. View

Nilsson K, GIOVANELLA B, STEHLIN J, Klein G . Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int J Cancer. 1977; 19(3):337-44. DOI: 10.1002/ijc.2910190309. View

Fresen K, Merkt B, Bornkamm G, Hausen H . Heterogeneity of Epstein-Barr virus originating from P3HR-1 cells. I. Studies on EBNA induction. Int J Cancer. 1977; 19(3):317-23. DOI: 10.1002/ijc.2910190306. View

Adams A, Lindahl T . Intracellular forms of Epstein-Barr virus DNA in Raji cells. IARC Sci Publ (1971). 1975; (11 Pt 1):125-32. View

Pater M, HYMAN R, Rapp F . Isolation of herpes simplex virus DNA from the "hirt supernatant". Virology. 1976; 75(2):481-3. DOI: 10.1016/0042-6822(76)90046-5. View

Botchan M, Topp W, Sambrook J . The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell. 1976; 9(2):269-87. DOI: 10.1016/0092-8674(76)90118-5. View

Fritsch E, Temin H . Formation and structure of infectious DNA of spleen necrosis virus. J Virol. 1977; 21(1):119-30. PMC: 353797. DOI: 10.1128/JVI.21.1.119-130.1977. View

10.

Groneberg J, Chardonnet Y, DOERFLER W . Integrated viral sequences in adenovirus type 12-transformed hamster cells. Cell. 1977; 10(1):101-11. DOI: 10.1016/0092-8674(77)90144-1. View

11.

Klein G, Lindahl T, Jondal M, Leibold W, Menezes J, Nilsson K . Continuous lymphoid cell lines with characteristics of B cells (bone-marrow-derived), lacking the Epstein-Barr virus genome and derived from three human lymphomas. Proc Natl Acad Sci U S A. 1974; 71(8):3283-6. PMC: 388669. DOI: 10.1073/pnas.71.8.3283. View

12.

Adams A, Lindahl T, Klein G . Linear association between cellular DNA and Epstein-Barr virus DNA in a human lymphoblastoid cell line. Proc Natl Acad Sci U S A. 1973; 70(10):2888-92. PMC: 427132. DOI: 10.1073/pnas.70.10.2888. View

13.

Epstein M, ACHONG B, BARR Y, Zajac B, Henle G, Henle W . Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji). J Natl Cancer Inst. 1966; 37(4):547-59. View

14.

Kersten W, Kersten H, SZYBALSKI W . Physicochemical properties of complexes between deoxyribonucleic acid and antibiotics which affect ribonucleic acid synthesis (actinomycin, daunomycin, cinerubin, nogalamycin, chormomycin, mithramycin, and olivomycin). Biochemistry. 1966; 5(1):236-44. DOI: 10.1021/bi00865a031. View

15.

Varmus H, Vogt P, Bishop J . Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts. Proc Natl Acad Sci U S A. 1973; 70(11):3067-71. PMC: 427171. DOI: 10.1073/pnas.70.11.3067. View

16.

Nonoyama M, Pagano J . Homology between Epstein-Barr virus DNA and viral DNA from Burkitt's lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics. Nature. 1973; 242(5392):44-7. DOI: 10.1038/242044a0. View

17.

Sambrook J, Westphal H, Srinivasan P, DULBECCO R . The integrated state of viral DNA in SV40-transformed cells. Proc Natl Acad Sci U S A. 1968; 60(4):1288-95. PMC: 224916. DOI: 10.1073/pnas.60.4.1288. View

18.

Hirt B . Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967; 26(2):365-9. DOI: 10.1016/0022-2836(67)90307-5. View

19.

Klein G, GIOVANELLA B, Westman A, STEHLIN J, Mumford D . An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology. 1975; 5(6):319-34. DOI: 10.1159/000149930. View

20.

Andersson M, Lindahl T . Epstein-Barr virus DNA in human lymphoid cell lines: in vitro conversion. Virology. 1976; 73(1):96-105. DOI: 10.1016/0042-6822(76)90064-7. View