Rapid Method for Isolation of Desiccation-tolerant Strains and Xeroprotectants

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2010 Jun 22

PMID 20562279

Citations 22

Authors

J J Narvaez-Reinaldo

I Barba

J Gonzalez-Lopez

A Tunnacliffe

M Manzanera

Affiliations

Soon will be listed here.

Abstract

A novel biotechnological process has been developed for the isolation of desiccation-tolerant microorganisms and their xeroprotectants, i.e., compatible solutes involved in long-term stability of biomolecules in the dry state. Following exposure of soil samples to chloroform, we isolated a collection of desiccation-tolerant microorganisms. This collection was screened for the production of xeroprotectants by a variation of the bacterial milking (osmotic downshock) procedure and by a novel air-drying/rehydration ("dry milking") incubation method. The resultant solutes were shown to protect both proteins and living cells against desiccation damage, thereby validating them as xeroprotectants. Nuclear magnetic resonance (NMR) analytical studies were performed to identify the xeroprotectants; synthetic mixtures of these compounds were shown to perform similarly to natural isolates in drying experiments with proteins and cells. This new approach has biotechnological and environmental implications for the identification of new xeroprotectants of commercial and therapeutic value.

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References

Wang W . Protein aggregation and its inhibition in biopharmaceutics. Int J Pharm. 2005; 289(1-2):1-30. DOI: 10.1016/j.ijpharm.2004.11.014. View

WALKER T, Han C, Kollman V, London R, Matwiyoff N . 13C nuclear magnetic resonance studies of the biosynthesis by Microbacterium ammoniaphilum of L-glutamate selectively enriched with carbon-13. J Biol Chem. 1982; 257(3):1189-95. View

Sauer T, Galinski E . Bacterial milking: A novel bioprocess for production of compatible solutes. Biotechnol Bioeng. 1999; 57(3):306-13. View

Zevenhuizen L . Levels of trehalose and glycogen in Arthrobacter globiformis under conditions of nutrient starvation and osmotic stress. Antonie Van Leeuwenhoek. 1992; 61(1):61-8. DOI: 10.1007/BF00572124. View

Manzanera M, Garcia de Castro A, Tondervik A, Strom A, Tunnacliffe A . Hydroxyectoine is superior to trehalose for anhydrobiotic engineering of Pseudomonas putida KT2440. Appl Environ Microbiol. 2002; 68(9):4328-33. PMC: 124095. DOI: 10.1128/AEM.68.9.4328-4333.2002. View

Bashan Y . Alginate beads as synthetic inoculant carriers for slow release of bacteria that affect plant growth. Appl Environ Microbiol. 1986; 51(5):1089-98. PMC: 239016. DOI: 10.1128/aem.51.5.1089-1098.1986. View

Manzanera M, Vilchez S, Tunnacliffe A . High survival and stability rates of Escherichia coli dried in hydroxyectoine. FEMS Microbiol Lett. 2004; 233(2):347-52. DOI: 10.1016/j.femsle.2004.03.005. View

SHULL J, CARGO G, Ernst R . KINETICS OF HEAT ACTIVATION AND OF THERMAL DEATH OF BACTERIAL SPORES. Appl Microbiol. 1963; 11:485-7. PMC: 1058035. DOI: 10.1128/am.11.6.485-487.1963. View

Arakawa T, Tsumoto K, Kita Y, Chang B, Ejima D . Biotechnology applications of amino acids in protein purification and formulations. Amino Acids. 2007; 33(4):587-605. DOI: 10.1007/s00726-007-0506-3. View

10.

Boylen C, Ensign J . Intracellular substrates for endogenous metabolism during long-term starvation of rod and spherical cells of Arthrobacter crystallopoietes. J Bacteriol. 1970; 103(3):578-87. PMC: 248129. DOI: 10.1128/jb.103.3.578-587.1970. View

11.

Manzanera M, Vilchez S, Tunnacliffe A . Plastic encapsulation of stabilized Escherichia coli and Pseudomonas putida. Appl Environ Microbiol. 2004; 70(5):3143-5. PMC: 404431. DOI: 10.1128/AEM.70.5.3143-3145.2004. View

12.

Yancey P, Clark M, Hand S, Bowlus R, Somero G . Living with water stress: evolution of osmolyte systems. Science. 1982; 217(4566):1214-22. DOI: 10.1126/science.7112124. View

13.

Alvarez H, Silva R, Cesari A, Zamit A, Peressutti S, Reichelt R . Physiological and morphological responses of the soil bacterium Rhodococcus opacus strain PD630 to water stress. FEMS Microbiol Ecol. 2009; 50(2):75-86. DOI: 10.1016/j.femsec.2004.06.002. View

14.

Carpenter J, Crowe L, Crowe J . Stabilization of phosphofructokinase with sugars during freeze-drying: characterization of enhanced protection in the presence of divalent cations. Biochim Biophys Acta. 1987; 923(1):109-15. DOI: 10.1016/0304-4165(87)90133-4. View

15.

Goyal K, Walton L, Tunnacliffe A . LEA proteins prevent protein aggregation due to water stress. Biochem J. 2005; 388(Pt 1):151-7. PMC: 1186703. DOI: 10.1042/BJ20041931. View

16.

Patro S, Freund E, Chang B . Protein formulation and fill-finish operations. Biotechnol Annu Rev. 2002; 8:55-84. DOI: 10.1016/s1387-2656(02)08004-3. View

17.

LeBlanc J, Goncalves E, Mohn W . Global response to desiccation stress in the soil actinomycete Rhodococcus jostii RHA1. Appl Environ Microbiol. 2008; 74(9):2627-36. PMC: 2394902. DOI: 10.1128/AEM.02711-07. View

18.

Gupta N, Rathi P, Gupta R . Simplified para-nitrophenyl palmitate assay for lipases and esterases. Anal Biochem. 2002; 311(1):98-9. DOI: 10.1016/s0003-2697(02)00379-2. View

19.

Tunnacliffe A, Garcia de Castro A, Manzanera M . Anhydrobiotic engineering of bacterial and mammalian cells: is intracellular trehalose sufficient?. Cryobiology. 2002; 43(2):124-32. DOI: 10.1006/cryo.2001.2356. View

20.

Franklin F, Bagdasarian M, Bagdasarian M, Timmis K . Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway. Proc Natl Acad Sci U S A. 1981; 78(12):7458-62. PMC: 349287. DOI: 10.1073/pnas.78.12.7458. View