Applications of Biological Pores in Nanomedicine, Sensing, and Nanoelectronics

Overview

Journal Curr Opin Biotechnol

Publisher Elsevier

Specialty Biotechnology

Date 2010 Jun 22

PMID 20561776

Citations 106

Authors

Sheereen Majd

Erik C Yusko

Yazan N Billeh

Michael X Macrae

Jerry Yang

Michael Mayer

Affiliations

Soon will be listed here.

Abstract

Biological protein pores and pore-forming peptides can generate a pathway for the flux of ions and other charged or polar molecules across cellular membranes. In nature, these nanopores have diverse and essential functions that range from maintaining cell homeostasis and participating in cell signaling to activating or killing cells. The combination of the nanoscale dimensions and sophisticated - often regulated - functionality of these biological pores make them particularly attractive for the growing field of nanobiotechnology. Applications range from single-molecule sensing to drug delivery and targeted killing of malignant cells. Potential future applications may include the use of nanopores for single strand DNA sequencing and for generating bio-inspired, and possibly, biocompatible visual detection systems and batteries. This article reviews the current state of applications of pore-forming peptides and proteins in nanomedicine, sensing, and nanoelectronics.

Citing Articles

Hetero-Oligomeric Protein Pores for Single-Molecule Sensing.

Satheesan R, Janeena A, Mahendran K J Membr Biol. 2024; .

PMID: 39699641 DOI: 10.1007/s00232-024-00331-2.

Computational Design of Pore-Forming Peptides with Potent Antimicrobial and Anticancer Activities.

Deb R, Torres M, Boudny M, Koberska M, Cappiello F, Popper M J Med Chem. 2024; 67(16):14040-14061.

PMID: 39116273 PMC: 11345766. DOI: 10.1021/acs.jmedchem.4c00912.

A highly sensitive nanopore platform for measuring RNase A activity.

Zheng H, Munusamy S, Arora P, Jahani R, Guan X Talanta. 2024; 276:126276.

PMID: 38796995 PMC: 11187776. DOI: 10.1016/j.talanta.2024.126276.

Deconvoluting the Effect of Cell-Penetrating Peptides for Enhanced and Controlled Insertion of Large-Scale DNA Nanopores.

Zhang X, Malle M, Thomsen R, Sorensen R, Sorensen E, Hatzakis N ACS Appl Mater Interfaces. 2024; 16(15):18422-18433.

PMID: 38573069 PMC: 11040531. DOI: 10.1021/acsami.3c18636.

T-S2Inet: Transformer-based sequence-to-image network for accurate nanopore sequence recognition.

Guan X, Shao W, Zhang D Bioinformatics. 2024; 40(2).

PMID: 38366607 PMC: 10902682. DOI: 10.1093/bioinformatics/btae083.

References

Meller A, Nivon L, Branton D . Voltage-driven DNA translocations through a nanopore. Phys Rev Lett. 2001; 86(15):3435-8. DOI: 10.1103/PhysRevLett.86.3435. View

Merrill D, Bikson M, Jefferys J . Electrical stimulation of excitable tissue: design of efficacious and safe protocols. J Neurosci Methods. 2005; 141(2):171-98. DOI: 10.1016/j.jneumeth.2004.10.020. View

Malmstadt N, Nash M, Purnell R, Schmidt J . Automated formation of lipid-bilayer membranes in a microfluidic device. Nano Lett. 2006; 6(9):1961-5. DOI: 10.1021/nl0611034. View

Poulos J, Jeon T, Damoiseaux R, Gillespie E, Bradley K, Schmidt J . Ion channel and toxin measurement using a high throughput lipid membrane platform. Biosens Bioelectron. 2008; 24(6):1806-10. DOI: 10.1016/j.bios.2008.08.041. View

Wolfe A, Mohammad M, Cheley S, Bayley H, Movileanu L . Catalyzing the translocation of polypeptides through attractive interactions. J Am Chem Soc. 2007; 129(45):14034-41. DOI: 10.1021/ja0749340. View

Banghart M, Volgraf M, Trauner D . Engineering light-gated ion channels. Biochemistry. 2006; 45(51):15129-41. DOI: 10.1021/bi0618058. View

Bechinger B . Structure and functions of channel-forming peptides: magainins, cecropins, melittin and alamethicin. J Membr Biol. 1997; 156(3):197-211. DOI: 10.1007/s002329900201. View

Borisenko V, Zhang Z, Woolley G . Gramicidin derivatives as membrane-based pH sensors. Biochim Biophys Acta. 2001; 1558(1):26-33. DOI: 10.1016/s0005-2736(01)00415-1. View

Apell H, Bamberg E, Lauger P . Effects of surface charge on the conductance of the gramicidin channel. Biochim Biophys Acta. 1979; 552(3):369-78. DOI: 10.1016/0005-2736(79)90181-0. View

10.

Kruse E, Uehlein N, Kaldenhoff R . The aquaporins. Genome Biol. 2006; 7(2):206. PMC: 1431727. DOI: 10.1186/gb-2006-7-2-206. View

11.

Rostovtseva T, Aguilella V, Vodyanoy I, Bezrukov S, Parsegian V . Membrane surface-charge titration probed by gramicidin A channel conductance. Biophys J. 1998; 75(4):1783-92. PMC: 1299850. DOI: 10.1016/S0006-3495(98)77620-9. View

12.

Movileanu L, Howorka S, Braha O, Bayley H . Detecting protein analytes that modulate transmembrane movement of a polymer chain within a single protein pore. Nat Biotechnol. 2000; 18(10):1091-5. DOI: 10.1038/80295. View

13.

Uram J, Mayer M . Estimation of solid phase affinity constants using resistive-pulses from functionalized nanoparticles. Biosens Bioelectron. 2006; 22(7):1556-60. DOI: 10.1016/j.bios.2006.06.020. View

14.

Montal M, Mueller P . Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc Natl Acad Sci U S A. 1972; 69(12):3561-6. PMC: 389821. DOI: 10.1073/pnas.69.12.3561. View

15.

Liu H, Qian S, Bau H . The effect of translocating cylindrical particles on the ionic current through a nanopore. Biophys J. 2006; 92(4):1164-77. PMC: 1783874. DOI: 10.1529/biophysj.106.089268. View

16.

Castellana E, Cremer P . Solid supported lipid bilayers: From biophysical studies to sensor design. Surf Sci Rep. 2020; 61(10):429-444. PMC: 7114318. DOI: 10.1016/j.surfrep.2006.06.001. View

17.

Bayley H, Martin C . Resistive-Pulse Sensing-From Microbes to Molecules. Chem Rev. 2001; 100(7):2575-2594. DOI: 10.1021/cr980099g. View

18.

Soletti R, de Faria G, Vernal J, Terenzi H, Anderluh G, Borges H . Potentiation of anticancer-drug cytotoxicity by sea anemone pore-forming proteins in human glioblastoma cells. Anticancer Drugs. 2008; 19(5):517-25. DOI: 10.1097/CAD.0b013e3282faa704. View

19.

Futaki S, Zhang Y, Kiwada T, Nakase I, Yagami T, Oiki S . Gramicidin-based channel systems for the detection of protein-ligand interaction. Bioorg Med Chem. 2004; 12(6):1343-50. DOI: 10.1016/j.bmc.2003.06.003. View

20.

Koper I . Insulating tethered bilayer lipid membranes to study membrane proteins. Mol Biosyst. 2007; 3(10):651-7. DOI: 10.1039/b707168j. View