Characterizing Proteins of Unknown Function: Orphan Cytochrome P450 Enzymes As a Paradigm

Overview

Journal Mol Interv

Specialty Molecular Biology

Date 2010 Jun 12

PMID 20539034

Citations 21

Authors

F Peter Guengerich

Zhongmei Tang

S Giovanna Salamanca-Pinzon

Qian Cheng

Affiliations

Soon will be listed here.

Abstract

With the rapid completion of genomic sequences of organisms today, we have far more gene products than functions we can ascribe. A number of experimental strategies have been developed and applied, both in vitro and in vivo, to put functions to these orphan proteins. The "deorphanization" of human and Streptomyces cytochrome P450 enzymes is considered quite important for pharmacology, with ramifications for the use of clinical therapeutics. The myriad of possibilities is too enormous to screen one reaction at a time, thus metabolomic or proteomic screens with complex biological samples are promising current strategies.

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