Notch Activity Levels Control the Balance Between Quiescence and Recruitment of Adult Neural Stem Cells

Overview

Journal J Neurosci

Specialty Neurology

Date 2010 Jun 11

PMID 20534844

Citations 120

Authors

Prisca Chapouton

Paulina Skupien

Birgit Hesl

Marion Coolen

John C Moore

Romain Madelaine

Elizabeth Kremmer

Theresa Faus-Kessler

Patrick Blader

Nathan D Lawson

Laure Bally-Cuif

Affiliations

Soon will be listed here.

Abstract

The limited generation of neurons during adulthood is controlled by a balance between quiescence and recruitment of neural stem cells (NSCs). We use here the germinal zone of the zebrafish adult telencephalon to examine how the frequency of NSC divisions is regulated. We show, using several in vivo techniques, that progenitors transit back and forth between the quiescent and dividing state, according to varying levels of Notch activity: Notch induction drives progenitors into quiescence, whereas blocking Notch massively reinitiates NSC division and subsequent commitment toward becoming neurons. Notch activation appears predominantly triggered by newly recruited progenitors onto their neighbors, suggesting an involvement of Notch in a self-limiting mechanism, once neurogenesis is started. These results identify for the first time a lateral inhibition-like mechanism in the context of adult neurogenesis and suggest that the equilibrium between quiescence and neurogenesis in the adult brain is controlled by fluctuations of Notch activity, thereby regulating the amount of adult-born neurons.

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