Changes in Apoptotic Gene Expression in Lymphocytes from Rheumatoid Arthritis and Systemic Lupus Erythematosus Patients Compared with Healthy Lymphocytes

Overview

Journal J Clin Immunol

Publisher Springer

Specialty Allergy & Immunology

Date 2010 Jun 10

PMID 20532967

Citations 11

Authors

Paul Eggleton

Lorna W Harries

Giada Alberigo

Paul Wordsworth

Nick Viner

Richard Haigh

Suzanne Donnelly

Hugh W Jones

Ian C Chikanza

Thomas W E OConner

Alasdair E R Thomson

Paul G Winyard

Affiliations

Soon will be listed here.

Abstract

Introduction: Systemic lupus erythematosus (SLE) and rheumatoid arthritis have complex genetic traits, but in both autoimmune diseases, dysfunctional apoptosis appears to play a part in disease pathology. This study examined the levels of in vitro apoptosis in lymphocytes from healthy, rheumatoid arthritis (RA) and SLE individuals and related observed differences to their lymphocyte apoptosis gene profiles.

Materials And Methods: Lymphocytes were assessed for cell death by nuclear pyknosis and DNA fragmentation. Control, SLE and RA apoptosis gene profiles were obtained by quantitative real-time polymerase chain reaction (QRT-PCR) analysis.

Results And Discussion: The mean levels of pyknosis in RA and SLE freshly isolated lymphocytes were significantly higher than in control lymphocytes. Ninety-three apoptosis genes were analysed by QRT-PCR of mRNA from RA, SLE and healthy lymphocytes. We identified significant differences (p < 0.05) in the expression of the same 11 of 93 and two of 93 apoptotic genes in individual SLE and RA patients tested as compared with controls.

Conclusion: We propose that similarly altered expression of specific apoptotic regulatory genes (e.g., the death effector domain-containing DNA-binding protein and apoptosis-associated speck-like protein containing a CARD) occurs in the lymphocytes of individual patients with SLE or RA that may influence the extent and rate of spontaneous apoptosis in these autoimmune conditions.

Citing Articles

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Baggio C, Luisetto R, Boscaro C, Scanu A, Ramonda R, Albiero M Int J Mol Sci. 2023; 24(6).

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Increased autophagy in CD4 T cells of rheumatoid arthritis patients results in T-cell hyperactivation and apoptosis resistance.

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