Molecular Markers Show a Complex Mosaic Pattern of Wheat-Thinopyrum Intermedium Translocations Carrying Resistance to YDV

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2010 Jun 8

PMID 20526575

Citations 2

Authors

Ligia Ayala-Navarrete

N Thompson

H Ohm

J Anderson

Affiliations

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Abstract

Thinopyrum intermedium translocations derived from the wheat (Triticum aestivum L.) substitution line P-29 were previously characterized by RFLP. We have further analyzed these lines and additional related germplasm with publicly available STS and SSRs. Primers which showed a polymorphism between wheat and P-29, were tested in all recombinant and nulli-tetrasomic lines confirming their position on chromosome 7D. The resulting 7D/7E chromosome maps appeared as a mosaic of wheat and Th. intermedium chromatin sections. To verify the composition of the translocation lines suggested by the RFLP-PCR map, F(2) progeny of two crosses (CS/216-1 and CS/260-1) were analyzed with molecular markers. Both populations gave an unexpectedly diverse number of recombinant individuals, suggesting that interstitial translocations occur more frequently than previously thought. This analysis also showed that there is a wide range in the number and position of the interstitial translocations within a given line such as the mosaic chromosome in recombinant line 260-1/CS-26, which has four Th. intermedium chromosome segments. Phenotypic data of the two populations suggested the presence of one gene which we have called Bdv3 to differentiate it from the previously reported orthologous gene Bdv2. Using the PCR-based molecular markers identified in this study, 5 out of 12 elite lines that showed good yields and no YDV symptoms contained Th. intermedium chromatin. Due to the multiple components involved in the YDV disease complex, combining selection for YDV resistance with the molecular markers and maps identified in this study will increase the efficiency of introgressing Th. intermedium chromatin containing YDV resistance or other beneficial traits into elite wheat germplasm.

Citing Articles

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References

Francki M, Crasta O, Sharma H, Ohm H, Anderson J . Structural organization of an alien Thinopyrum intermedium group 7 chromosome in U.S. soft red winter wheat (Triticum aestivum L.). Genome. 2008; 40(5):716-22. DOI: 10.1139/g97-794. View

West C, Waterworth W, Story G, Sunderland P, Jiang Q, Bray C . Disruption of the Arabidopsis AtKu80 gene demonstrates an essential role for AtKu80 protein in efficient repair of DNA double-strand breaks in vivo. Plant J. 2002; 31(4):517-28. DOI: 10.1046/j.1365-313x.2002.01370.x. View

Pestsova E, Ganal M, Roder M . Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome. 2000; 43(4):689-97. View

Roder M, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P . A microsatellite map of wheat. Genetics. 1998; 149(4):2007-23. PMC: 1460256. DOI: 10.1093/genetics/149.4.2007. View

Crasta O, Francki M, Bucholtz D, Sharma H, Zhang J, Wang R . Identification and characterization of wheat-wheatgrass translocation lines and localization of barley yellow dwarf virus resistance. Genome. 2000; 43(4):698-706. View

Sharma H, Ohm H, Goulart L, Lister R, Appels R, Benlhabib O . Introgression and characterization of barley yellow dwarf virus resistance from Thinopyrum intermedium into wheat. Genome. 1995; 38(2):406-13. DOI: 10.1139/g95-052. View

Morgan W, Hartmann A, Limoli C, Nagar S, Ponnaiya B . Bystander effects in radiation-induced genomic instability. Mutat Res. 2002; 504(1-2):91-100. DOI: 10.1016/s0027-5107(02)00083-0. View

Jiang J, Gill B . A 'zebra' chromosome arising from multiple translocations involving non-homologous chromosomes. Chromosoma. 1993; 102(9):612-7. DOI: 10.1007/BF00352308. View

Chen Q, Conner R, Laroche A, Thomas J . Genome analysis of Thinopyrum intermedium and Thinopyrum ponticum using genomic in situ hybridization. Genome. 1998; 41(4):580-6. View

10.

Larkin P, Banks P, Lagudah E, Appels R, Xiao C, Zhiyong X . Disomic Thinopyrum intermedium addition lines in wheat with barley yellow dwarf virus resistance and with rust resistances. Genome. 1995; 38(2):385-94. DOI: 10.1139/g95-050. View

11.

Banks P, Xu S, Wang R, Larkin P . Varying chromosome composition of 56-chromosome wheat x Thinopyrum intermedium partial amphiploids. Genome. 1993; 36(2):207-15. DOI: 10.1139/g93-029. View

12.

Anderson J, Bucholtz D, GREENE A, Francki M, Gray S, Sharma H . Characterization of wheatgrass-derived barley yellow dwarf virus resistance in a wheat alien chromosome substitution line. Phytopathology. 2008; 88(8):851-5. DOI: 10.1094/PHYTO.1998.88.8.851. View

13.

Soliman K, Jorgensen R, Allard R . Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci U S A. 1984; 81(24):8014-8. PMC: 392284. DOI: 10.1073/pnas.81.24.8014. View

14.

Britt . Molecular genetics of DNA repair in higher plants. Trends Plant Sci. 1999; 4(1):20-25. DOI: 10.1016/s1360-1385(98)01355-7. View

15.

Ayala-Navarrete L, Bariana H, Singh R, Gibson J, Mechanicos A, Larkin P . Trigenomic chromosomes by recombination of Thinopyrum intermedium and Th. ponticum translocations in wheat. Theor Appl Genet. 2007; 116(1):63-75. DOI: 10.1007/s00122-007-0647-5. View

16.

Roder M, Plaschke J, Konig S, Borner A, Sorrells M, Tanksley S . Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet. 1995; 246(3):327-33. DOI: 10.1007/BF00288605. View

17.

Zhang Z, Lin Z, Xin Z . Research progress in BYDV resistance genes derived from wheat and its wild relatives. J Genet Genomics. 2009; 36(9):567-73. DOI: 10.1016/S1673-8527(08)60148-4. View

18.

Wright E, Coates P . Untargeted effects of ionizing radiation: implications for radiation pathology. Mutat Res. 2006; 597(1-2):119-32. DOI: 10.1016/j.mrfmmm.2005.03.035. View

19.

Xin Z, Velten J, Oliver M, Burke J . High-throughput DNA extraction method suitable for PCR. Biotechniques. 2003; 34(4):820-4, 826. DOI: 10.2144/03344rr04. View

20.

Sharma H, Ohm H, Lister R, Foster J, Shukle R . Response of wheatgrasses and wheat × wheatgrass hybrids to barley yellow dwarf virus. Theor Appl Genet. 2013; 77(3):369-74. DOI: 10.1007/BF00305830. View