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Fabrication of Elastomer Pillar Arrays with Modulated Stiffness for Cellular Force Measurements

Overview
Journal J Vac Sci Technol B Microelectron Nanometer Struct Process Meas Phenom
Date 2011 Sep 28
PMID 20526426
Citations 15
Authors
S Ghassemi
N Biais
K Maniura
S J Wind
M P Sheetz
J Hone
Affiliations
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Abstract

The mechanical properties of a cell's environment can alter behavior such as migration and spreading, and control the differentiation path of stem cells. Here we describe a technique for fabricating substrates whose rigidity can be controlled locally without altering the contact area for cell spreading. The substrates consist of elastomeric pillar arrays in which the top surface is uniform but the pillar height is changed across a sharp step. Preliminary results demonstrate the effects on cell migration and morphology at the step boundary.

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