Characterization of Conformation-specific Monoclonal Antibodies Against Rabies Virus Nucleoprotein

Overview

Journal Arch Virol

Specialty Microbiology

Date 2010 Jun 4

PMID 20521069

Citations 12

Authors

Yan Jiang

Yonghuang Luo

Frank Michel

Robert J Hogan

Ying He

Zhen F Fu

Affiliations

Soon will be listed here.

Abstract

Three anti-rabies virus (RABV) nucleoprotein (N) monoclonal antibodies (Mab) were characterized by immunofluorescence assays, western blotting, and immunohistochemistry. One of these Mabs recognized the antigen by all of the assays, while the other two recognized N only in the native form in the immunofluorescence assay. These data, together with epitope mapping studies, suggest that two anti-N Mabs recognize conformational epitopes located within the N-terminal region of the RABV N protein. The availability of Mabs specific for both linear and epitope-specific antibodies should prove valuable for rabies diagnosis as well as for RABV N protein structure-function studies.

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