Ecological Temporal Stability of Staphylococcus Aureus Nasal Carriage

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2010 Jun 4

PMID 20519475

Citations 20

Authors

Olga Sakwinska

Dominique S Blanc

Catherine Lazor-Blanchet

Martine Moreillon

Marlyse Giddey

Philippe Moreillon

Affiliations

Soon will be listed here.

Abstract

We described the colonization dynamics of Staphylococcus aureus in a group of 266 healthy carriers over a period of approximately 1 year. We used precise genotyping methods, i.e., amplified fragment length polymorphism (AFLP), spa typing, and double-locus sequence typing (DLST), to detect changes in strain identity. Strain change took place rather rarely: out of 89 carriers who had initially been colonized, only 7 acquired a strain different from the original one. Approximately one-third of the carriers eliminated the colonization, and a similar number became newly colonized. Some of these events probably represent detection failure rather than genuine colonization loss or acquisition. Lower bacterial counts were associated with increased probability of eliminating the colonization. We have confirmed a high mutation rate in the spa locus: 6 out of 53 strains underwent mutation in the spa locus. There was no overall change in S. aureus genotype composition.

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