Demonstration and Partial Characterization of a Bacterial Growth Enhancer in Sera

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2010 Jun 2

PMID 20514485

Authors

Kanna Okayama

Takeshi Honda

Shigeaki Matsuda

Tadashi Saito

Masaya Kawase

Affiliations

Soon will be listed here.

Abstract

During our research into the pathogenesis of Vibrio parahaemolyticus, we noticed that the concentration of serum added to the tissue culture medium (Dulbecco's modified Eagle's medium: DMEM) greatly affected its growth. Using gel filtration column chromatography, we clearly demonstrated that serum contains not only a bacterial growth inhibitor (BGI) but also a bacterial growth enhancer (BGE) for Vibrio parahaemolyticus. Our data indicate that the BGI is transferrin, whereas the BGE seems to be an undescribed small molecule (molecular weight of 1,000-3,000 Da) and is associated with magnesium and molybdenum ions. BGE activity was not decreased by heat treatment (at 60 or 100°C for 30 min) and affected the growth rate of a wide range of Gram-negative and Gram-positive bacteria. The addition of EDTA into DMEM lowered the growth rate, whereas the addition of BGE restored the growth activity. This study suggests that sera contain a previously undescribed small BGE molecule.

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