Expanding the Mouse Embryonic Stem Cell Proteome: Combining Three Proteomic Approaches

Overview

Journal Proteomics

Date 2010 Jun 1

PMID 20512790

Citations 13

Authors

Rebekah L Gundry

Irina Tchernyshyov

Shijun Sheng

Yelena Tarasova

Kimberly Raginski

Kenneth R Boheler

Jennifer E Van Eyk

Affiliations

Soon will be listed here.

Abstract

The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic digestion and subsequent analysis by MS. The results yielded 1895 identified non-redundant proteins and, for 128 of these, the specific isoform could be determined based on detection of an isoform-specific peptide. When compared with two previously published proteomic studies that used the same cell line, the current study reveals 612 new proteins.

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