The Archaeo-eukaryotic Primase of Plasmid PRN1 Requires a Helix Bundle Domain for Faithful Primer Synthesis

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2010 Jun 1

PMID 20511586

Citations 18

Authors

Kirsten Beck

Alessandro Vannini

Patrick Cramer

Georg Lipps

Affiliations

Soon will be listed here.

Abstract

The plasmid pRN1 encodes for a multifunctional replication protein with primase, DNA polymerase and helicase activity. The minimal region required for primase activity encompasses amino-acid residues 40-370. While the N-terminal part of that minimal region (residues 47-247) folds into the prim/pol domain and bears the active site, the structure and function of the C-terminal part (residues 248-370) is unknown. Here we show that the C-terminal part of the minimal region folds into a compact domain with six helices and is stabilized by a disulfide bond. Three helices superimpose well with the C-terminal domain of the primase of the bacterial broad host range plasmid RSF1010. Structure-based site-directed mutagenesis shows that the C-terminal helix of the helix bundle domain is required for primase activity although it is distant to the active site in the crystallized conformation. Furthermore, we identified mutants of the C-terminal domain, which are defective in template binding, dinucleotide formation and conformation change prior to DNA extension.

Citing Articles

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Stringent Primer Termination by an Archaeo-Eukaryotic DNA Primase.

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