Osteopenia in the Maxillofacial Area: a Study in Sheep

Overview

Journal Osteoporos Int

Specialties Endocrinology
Orthopedics

Date 2010 May 27

PMID 20503036

Citations 8

Authors

E Veigel

R J Moore

M R Zarrinkalam

D Schulze

S Sauerbier

R Schmelzeisen

P J Voss

Affiliations

Soon will be listed here.

Abstract

Unlabelled: Osteoporosis is a major public health problem worldwide. Its significance in the fields of traumatology and implantology of the maxillofacial area requires investigation. A large animal model was used to assess bone loss in the lumbar spine and mandible.

Introduction: Osteoporosis is a prevalent disease characterized by low bone mass and microarchitectural deterioration of bone tissue. Osteoporosis-related fractures represent a major public health burden. The presence and relevance of osteoporosis in the maxillofacial area remain controversial. Research in humans is limited by difficulties in finding large homogenous study groups and, due to ethical considerations, numerous animal models have been used in osteoporosis research. The aim of this study was to assess a sheep model of generalized osteopenia for changes in the maxillofacial area.

Methods: Bone loss was induced in ten Merino sheep by ovariectomy, intramuscular administration of glucocorticoids, and a calcium-reduced diet. Five untreated animals served as controls. Bone mineral density (BMD) was assessed by dual-energy X-ray absorptiometry at the lumbar spine at baseline and after 1, 3, 6, 9, and 12 months. Lumbar and mandibular bone biopsies were obtained and analyzed with microcomputed tomography.

Results: Lumbar BMD decreased progressively in the intervention group and was most significantly low after 6 months (p < 0.001). Lumbar trabecular bone showed a significant decrease in bone volume (BV)/tissue volume (TV; p < 0.05) in the inducted group. Significant changes were found in both analyzed mandibular regions for BV/TV (p < 0.05). Regional variations were found for other parameters in the mandible. The cortical width was substantially reduced in the intervention group (p < 0.001).

Conclusions: Microstructural changes occurring in sheep as a result of induction seem to have a generalized nature. This sheep model meets the criteria for further investigation in the maxillofacial area.

Citing Articles

Glucocorticoids affect bone mineral density and bone remodelling in OVX sheep: A pilot study.

Cabrera D, Wolber F, Dittmer K, Rogers C, Ridler A, Aberdein D Bone Rep. 2018; 9:173-180.

PMID: 30480061 PMC: 6249392. DOI: 10.1016/j.bonr.2018.11.001.

Preclinical and Translational Studies in Small Ruminants (Sheep and Goat) as Models for Osteoporosis Research.

Dias I, Camassa J, Bordelo J, Babo P, Viegas C, Dourado N Curr Osteoporos Rep. 2018; 16(2):182-197.

PMID: 29460175 DOI: 10.1007/s11914-018-0431-2.

Site-Specific Variations in Bone Mineral Density under Systemic Conditions Inducing Osteoporosis in Minipigs.

Schulz M, Kowald J, Estenfelder S, Jung R, Kuhlisch E, Eckelt U Front Physiol. 2017; 8:426.

PMID: 28676766 PMC: 5476774. DOI: 10.3389/fphys.2017.00426.

Evaluation of BP-ONJ in osteopenic and healthy sheep: comparing ZTE-MRI with µCT.

Voss P, Ludwig U, Poxleitner P, Bergmaier V, El-Shafi N, von Elverfeldt D Dentomaxillofac Radiol. 2016; 45(4):20150250.

PMID: 26846710 PMC: 4846170. DOI: 10.1259/dmfr.20150250.

Zoledronate induces bisphosphonate-related osteonecrosis of the jaw in osteopenic sheep.

Voss P, Stoddart M, Bernstein A, Schmelzeisen R, Nelson K, Stadelmann V Clin Oral Investig. 2015; 20(1):31-8.

PMID: 25843053 DOI: 10.1007/s00784-015-1468-6.

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