P2X and NMDA Receptor Involvement in Temporomandibular Joint-evoked Reflex Activity in Rat Jaw Muscles

Overview

Journal Brain Res

Specialty Neurology

Date 2010 May 27

PMID 20501327

Citations 4

Authors

T Watanabe

Y Tsuboi

B J Sessle

K Iwata

J W Hu

Affiliations

Soon will be listed here.

Abstract

We have previously shown that injection of the excitatory amino glutamate into the rat temporomandibular joint (TMJ) evokes reflex activity in both anterior digastric (DIG) and masseter (MASS) muscles that can be attenuated by prior TMJ injection of an N-methyl-d-aspartate (NMDA) receptor antagonist. The aim of the present study was to test if jaw muscle activity could also be evoked by P2X receptor agonist injection into the rat TMJ region and if the reflex activity could be modulated by TMJ injection of P2X receptor antagonist or NMDA receptor antagonist. The selective P2X subtype agonist alpha,beta-methylene adenosine 5'-triphosphate (alpha,beta-me ATP) and vehicle (phosphate-buffered saline) or the selective P2X antagonist, 2'-(or-3')-O-(2,4,6-trinitrophenyl) adenosine 5'-triphosphate (TNP-ATP) or the selective NMDA antagonist (+/-)-d-2-amino-5-phosphonovalerate(APV) were injected into the rat TMJ region. Electromyographic (EMG) reflex activity was recorded in both DIG and MASS muscles. Compared with the baseline EMG activity, alpha,beta-me-ATP injection into the TMJ (but not its systemic administration) following pre-injection of the vehicle significantly increased the magnitude and the duration of ipsilateral DIG and MASS EMG activity in a dose-dependent manner. The alpha,beta-me-ATP-evoked responses could be antagonized by pre-injection of TNP-ATP into the same TMJ site but contralateral TMJ injection of TNP-ATP proved ineffective. Furthermore, the alpha,beta-me-ATP-evoked responses could also be antagonized by APV injected into the same TMJ site but not by its systemic injection. These results indicate the interaction of peripheral purinergic as well as glutamatergic receptor mechanisms in the processing of TMJ nociceptive afferent inputs that evoke reflex activity in jaw muscles.

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References

Ambalavanar R, Moritani M, Dessem D . Trigeminal P2X3 receptor expression differs from dorsal root ganglion and is modulated by deep tissue inflammation. Pain. 2005; 117(3):280-291. DOI: 10.1016/j.pain.2005.06.029. View

Ichikawa H, Fukunaga T, Jin H, Fujita M, Takano-Yamamoto T, Sugimoto T . VR1-, VRL-1- and P2X3 receptor-immunoreactive innervation of the rat temporomandibular joint. Brain Res. 2004; 1008(1):131-6. DOI: 10.1016/j.brainres.2004.02.029. View

Gu J, Macdermott A . Activation of ATP P2X receptors elicits glutamate release from sensory neuron synapses. Nature. 1997; 389(6652):749-53. DOI: 10.1038/39639. View

Macdermott A, Role L, Siegelbaum S . Presynaptic ionotropic receptors and the control of transmitter release. Annu Rev Neurosci. 1999; 22:443-85. DOI: 10.1146/annurev.neuro.22.1.443. View

Petruska J, Cooper B, Gu J, Rau K, Johnson R . Distribution of P2X1, P2X2, and P2X3 receptor subunits in rat primary afferents: relation to population markers and specific cell types. J Chem Neuroanat. 2000; 20(2):141-62. DOI: 10.1016/s0891-0618(00)00080-6. View

Shinoda M, Ozaki N, Asai H, Nagamine K, Sugiura Y . Changes in P2X3 receptor expression in the trigeminal ganglion following monoarthritis of the temporomandibular joint in rats. Pain. 2005; 116(1-2):42-51. DOI: 10.1016/j.pain.2005.03.042. View

Tashiro A, Okamoto K, Bereiter D . Morphine modulation of temporomandibular joint-responsive units in superficial laminae at the spinomedullary junction in female rats depends on estrogen status. Eur J Neurosci. 2008; 28(10):2065-74. DOI: 10.1111/j.1460-9568.2008.06488.x. View

North R . P2X3 receptors and peripheral pain mechanisms. J Physiol. 2003; 554(Pt 2):301-8. PMC: 1664762. DOI: 10.1113/jphysiol.2003.048587. View

Tsai C, Chiang C, Yu X, Sessle B . Involvement of trigeminal subnucleus caudalis (medullary dorsal horn) in craniofacial nociceptive reflex activity. Pain. 1999; 81(1-2):115-28. DOI: 10.1016/s0304-3959(99)00009-3. View

10.

Khakh B, Burnstock G, Kennedy C, King B, North R, Seguela P . International union of pharmacology. XXIV. Current status of the nomenclature and properties of P2X receptors and their subunits. Pharmacol Rev. 2001; 53(1):107-18. View

11.

Jarvis M . The neural-glial purinergic receptor ensemble in chronic pain states. Trends Neurosci. 2009; 33(1):48-57. DOI: 10.1016/j.tins.2009.10.003. View

12.

Sessle J, Haas A, Izzo A, Vernon H, Hu W . Involvement of NMDA receptor mechanisms in jaw electromyographic activity and plasma extravasation induced by inflammatory irritant application to temporomandibular joint region of rats. Pain. 1996; 68(1):169-178. DOI: 10.1016/S0304-3959(96)03181-8. View

13.

Cairns B, Sessle B, Hu J . Evidence that excitatory amino acid receptors within the temporomandibular joint region are involved in the reflex activation of the jaw muscles. J Neurosci. 1998; 18(19):8056-64. PMC: 6792993. View

14.

Lam D, Sessle B, Hu J . Glutamate and capsaicin effects on trigeminal nociception II: activation and central sensitization in brainstem neurons with deep craniofacial afferent input. Brain Res. 2008; 1253:48-59. DOI: 10.1016/j.brainres.2008.11.056. View

15.

Bakke M, Hu J, Sessle B . Involvement of NK-1 and NK-2 tachykinin receptor mechanisms in jaw muscle activity reflexly evoked by inflammatory irritant application to the rat temporomandibular joint. Pain. 1998; 75(2-3):219-27. DOI: 10.1016/s0304-3959(97)00223-6. View

16.

Kim Y, Paik S, Cho Y, Shin H, Bae J, Moritani M . Expression of P2X3 receptor in the trigeminal sensory nuclei of the rat. J Comp Neurol. 2007; 506(4):627-39. DOI: 10.1002/cne.21544. View

17.

Tashiro A, Okamoto K, Milam S, Bereiter D . Differential effects of estradiol on encoding properties of TMJ units in laminae I and V at the spinomedullary junction in female rats. J Neurophysiol. 2007; 98(6):3242-53. DOI: 10.1152/jn.00677.2007. View

18.

Surprenant A, North R . Signaling at purinergic P2X receptors. Annu Rev Physiol. 2008; 71:333-59. DOI: 10.1146/annurev.physiol.70.113006.100630. View

19.

Burnstock G . Physiology and pathophysiology of purinergic neurotransmission. Physiol Rev. 2007; 87(2):659-797. DOI: 10.1152/physrev.00043.2006. View

20.

Vulchanova L, Riedl M, Shuster S, Buell G, Surprenant A, North R . Immunohistochemical study of the P2X2 and P2X3 receptor subunits in rat and monkey sensory neurons and their central terminals. Neuropharmacology. 1997; 36(9):1229-42. DOI: 10.1016/s0028-3908(97)00126-3. View