Reconstruction of the UDP-N-acetylglucosamine Biosynthetic Pathway in Cell-free System

Overview

Journal Biotechnol Lett

Specialties Biochemistry
Biotechnology

Date 2010 May 25

PMID 20495944

Citations 5

Authors

Jiewen Zhou

Lei Huang

Jiazhang Lian

Jiayuan Sheng

Jin Cai

Zhinan Xu

Affiliations

Soon will be listed here.

Abstract

A functional glucokinase (YqgR), N-acetylglucosamine-phosphate mutase (Agm1) and N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) were synthesized simultaneously in an Escherichia coli cell-free system. These three enzymes were coupled to reconstruct a biosynthetic pathway which could produce UDP-N-acetylglucosamine with N-acetylglucosamine as the substrate.

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