P2X7 Receptor-mediated Killing of an Intracellular Parasite, Toxoplasma Gondii, by Human and Murine Macrophages

Overview

Journal J Immunol

Specialty Allergy & Immunology

Date 2010 May 22

PMID 20488797

Citations 69

Authors

Michael P Lees

Stephen J Fuller

Rima McLeod

Nicola R Boulter

Catherine M Miller

Alana M Zakrzewski

Ernest J Mui

William H Witola

Jessica J Coyne

Aubrey C Hargrave

Sarra E Jamieson

Jenefer M Blackwell

James S Wiley

Nicholas C Smith

Affiliations

Soon will be listed here.

Abstract

The P2X7R is highly expressed on the macrophage cell surface, and activation of infected cells by extracellular ATP has been shown to kill intracellular bacteria and parasites. Furthermore, single nucleotide polymorphisms that decrease receptor function reduce the ability of human macrophages to kill Mycobacterium tuberculosis and are associated with extrapulmonary tuberculosis. In this study, we show that macrophages from people with the 1513C (rs3751143, NM_002562.4:c.1487A>C) loss-of-function P2X7R single nucleotide polymorphism are less effective in killing intracellular Toxoplasma gondii after exposure to ATP compared with macrophages from people with the 1513A wild-type allele. Supporting a P2X7R-specific effect on T. gondii, macrophages from P2X7R knockout mice (P2X7R-/-) are unable to kill T. gondii as effectively as macrophages from wild-type mice. We show that P2X7R-mediated T. gondii killing occurs in parallel with host cell apoptosis and is independent of NO production.

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