Study of Potential Inhibitors of Thyroid Iodide Uptake by Using CHO Cells Stably Expressing the Human Sodium/iodide Symporter (hNIS) Protein

Overview

Journal J Endocrinol Invest

Publisher Springer

Specialty Endocrinology

Date 2010 May 19

PMID 20479570

Citations 1

Authors

P Agretti

A Dimida

G De Marco

E Ferrarini

J C Rodriguez Gonzalez

F Santini

P Vitti

A Pinchera

M Tonacchera

Affiliations

Soon will be listed here.

Abstract

Background: Thyroid gland is highly dependent on dietary intake of iodine for normal function, so it is particularly subjected to "endocrine disruptor" action. The human sodium/iodide symporter (hNIS) is an integral plasma membrane glycoprotein mediating the active transport of iodide into thyroid follicular cells, a crucial step for thyroid hormone biosynthesis. Beyond to perchlorate and thyocianate ions a few other inhibitors of iodide uptake have been described.

Aim: The aim of this study was to investigate if 10 substances usually used as drugs in clinical practice were able to inhibit NIS-mediated iodide uptake in vitro.

Materials And Methods: A CHO cell line stably expressing hNIS was used to test any inhibition of NIS-mediated iodide uptake exerted by drugs. Perchlorate and thyocianate ions were used as positive controls.

Results: None of the analyzed substances was able to significantly inhibit iodide uptake in our system. As we expected, perchlorate and thyocianate ions were able to inhibit iodide uptake in a dose-dependent manner.

Conclusions: In conclusion, we carried out an in vitro assay to evaluate the potential inhibitory effect of common drugs on NISmediated iodide uptake by using CHO-hNIS cells. None of the analyzed substances was able to inhibit iodide uptake; only perchlorate and thyocianate were able to inhibit iodide uptake in a dose-dependent manner.

Citing Articles

Fluorescence Sensing of the Interaction between Biomembranes with Different Lipid Composition and Endocrine Disrupting Chemicals.

Nakane Y, Kubo I Materials (Basel). 2017; 7(1):170-179.

PMID: 28788448 PMC: 5453125. DOI: 10.3390/ma7010170.

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