Atypical Protein Kinase C Expression in Phrenic Motor Neurons of the Rat

Overview

Journal Neuroscience

Specialty Neurology

Date 2010 May 19

PMID 20478365

Citations 10

Authors

C H Guenther

S Vinit

J A Windelborn

M Behan

G S Mitchell

Affiliations

Soon will be listed here.

Abstract

Atypical protein kinase C (PKC) isoforms play important roles in many neural processes, including synaptic plasticity and neurodegenerative diseases. Although atypical PKCs are expressed throughout the brain, there are no reports concerning their expression in central neural regions associated with respiratory motor control. Therefore, we explored the neuroanatomical distribution of atypical PKCs in identified phrenic motor neurons, a motor pool that plays a key role in breathing. Diaphragm injections of cholera toxin B were used to retrogradely label and identify phrenic motor neurons; immunohistochemistry was used to localize atypical PKCs in and near labeled motor neurons (i.e. the phrenic motor nucleus). Atypical PKC expression in the phrenic motor nucleus appears specific to neurons; aPKC expression could not be detected in adjacent astrocytes or microglia. Strong atypical PKC labeling was observed within cholera toxin B labeled phrenic motor neurons. Documenting the expression of atypical PKCs in phrenic motor neurons provides a framework within which to assess their role in respiratory motor control, including novel forms of respiratory plasticity known to occur in this region.

Citing Articles

Serotonergic innervation of respiratory motor nuclei after cervical spinal injury: Impact of intermittent hypoxia.

Ciesla M, Seven Y, Allen L, Smith K, Asa Z, Simon A Exp Neurol. 2021; 338:113609.

PMID: 33460645 PMC: 8327480. DOI: 10.1016/j.expneurol.2021.113609.

aPKC in neuronal differentiation, maturation and function.

Hapak S, Rothlin C, Ghosh S Neuronal Signal. 2020; 3(3):NS20190019.

PMID: 32269838 PMC: 7104321. DOI: 10.1042/NS20190019.

Spinal protein phosphatase 1 constrains respiratory plasticity after sustained hypoxia.

Huxtable A, Peterson T, Ouellette J, Watters J, Mitchell G J Appl Physiol (1985). 2018; 125(5):1440-1446.

PMID: 30161006 PMC: 6295481. DOI: 10.1152/japplphysiol.00641.2018.

Cyclooxygenase enzyme activity does not impair respiratory motor plasticity after one night of intermittent hypoxia.

Huxtable A, Kopp E, Dougherty B, Watters J, Mitchell G Respir Physiol Neurobiol. 2017; 256:21-28.

PMID: 29233741 PMC: 5994173. DOI: 10.1016/j.resp.2017.12.004.

Plasticity in respiratory motor neurons in response to reduced synaptic inputs: A form of homeostatic plasticity in respiratory control?.

Braegelmann K, Streeter K, Fields D, Baker T Exp Neurol. 2016; 287(Pt 2):225-234.

PMID: 27456270 PMC: 5683174. DOI: 10.1016/j.expneurol.2016.07.012.

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