Atomic Force Microscopy Probing in the Measurement of Cell Mechanics

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2010 May 14

PMID 20463929

Citations 48

Authors

Dimitrios Kirmizis

Stergios Logothetidis

Affiliations

Soon will be listed here.

Abstract

Atomic force microscope (AFM) has been used incrementally over the last decade in cell biology. Beyond its usefulness in high resolution imaging, AFM also has unique capabilities for probing the viscoelastic properties of living cells in culture and, even more, mapping the spatial distribution of cell mechanical properties, providing thus an indirect indicator of the structure and function of the underlying cytoskeleton and cell organelles. AFM measurements have boosted our understanding of cell mechanics in normal and diseased states and provide future potential in the study of disease pathophysiology and in the establishment of novel diagnostic and treatment options.

Citing Articles

Atomic Force Microscopy of Viruses.

de Pablo P Subcell Biochem. 2024; 105:329-357.

PMID: 39738951 DOI: 10.1007/978-3-031-65187-8_9.

Role of adhesion in the mechanics of pathogenic Listeria monocytogenes EGDe as a function of the pH of growth.

Eskhan A, Abu-Lail N Biointerphases. 2024; 19(5).

PMID: 39441073 PMC: 11501791. DOI: 10.1116/6.0003840.

Mechanical deformation and death of circulating tumor cells in the bloodstream.

Qiu Y, Gao T, Smith B Cancer Metastasis Rev. 2024; 43(4):1489-1510.

PMID: 38980581 PMC: 11900898. DOI: 10.1007/s10555-024-10198-3.

A method for analyzing AFM force mapping data obtained from soft tissue cryosections.

Wong C, Fraticelli Guzman N, Read A, Hedberg-Buenz A, Anderson M, Feola A J Biomech. 2024; 168:112113.

PMID: 38648717 PMC: 11128031. DOI: 10.1016/j.jbiomech.2024.112113.

Advanced Mechanical Testing Technologies at the Cellular Level: The Mechanisms and Application in Tissue Engineering.

Zhu Y, Zhang M, Sun Q, Wang X, Li X, Li Q Polymers (Basel). 2023; 15(15).

PMID: 37571149 PMC: 10422338. DOI: 10.3390/polym15153255.

References

Dufrene Y . Towards nanomicrobiology using atomic force microscopy. Nat Rev Microbiol. 2008; 6(9):674-80. DOI: 10.1038/nrmicro1948. View

Mahaffy R, Shih C, MacKintosh F, Kas J . Scanning probe-based frequency-dependent microrheology of polymer gels and biological cells. Phys Rev Lett. 2000; 85(4):880-3. DOI: 10.1103/PhysRevLett.85.880. View

Heidemann S, Kaech S, Buxbaum R, MATUS A . Direct observations of the mechanical behaviors of the cytoskeleton in living fibroblasts. J Cell Biol. 1999; 145(1):109-22. PMC: 2148213. DOI: 10.1083/jcb.145.1.109. View

Cross S, Jin Y, Tondre J, Wong R, Rao J, Gimzewski J . AFM-based analysis of human metastatic cancer cells. Nanotechnology. 2011; 19(38):384003. DOI: 10.1088/0957-4484/19/38/384003. View

DVORAK J, Nagao E . Kinetic analysis of the mitotic cycle of living vertebrate cells by atomic force microscopy. Exp Cell Res. 1998; 242(1):69-74. DOI: 10.1006/excr.1998.4077. View

Rotsch C, Radmacher M . Drug-induced changes of cytoskeletal structure and mechanics in fibroblasts: an atomic force microscopy study. Biophys J. 2000; 78(1):520-35. PMC: 1300659. DOI: 10.1016/S0006-3495(00)76614-8. View

Cross S, Jin Y, Rao J, Gimzewski J . Nanomechanical analysis of cells from cancer patients. Nat Nanotechnol. 2008; 2(12):780-3. DOI: 10.1038/nnano.2007.388. View

Wautier J, Schmid-Schonbein G, Nash G . Measurement of leukocyte rheology in vascular disease: clinical rationale and methodology. International Society of Clinical Hemorheology. Clin Hemorheol Microcirc. 1999; 21(1):7-24. View

McElfresh M, Baesu E, Balhorn R, Belak J, Allen M, Rudd R . Combining constitutive materials modeling with atomic force microscopy to understand the mechanical properties of living cells. Proc Natl Acad Sci U S A. 2002; 99 Suppl 2:6493-7. PMC: 128556. DOI: 10.1073/pnas.082520599. View

10.

Nagao E, DVORAK J . Phase imaging by atomic force microscopy: analysis of living homoiothermic vertebrate cells. Biophys J. 1999; 76(6):3289-97. PMC: 1300298. DOI: 10.1016/S0006-3495(99)77481-3. View

11.

Jones W, Ting-Beall H, Lee G, Kelley S, Hochmuth R, Guilak F . Alterations in the Young's modulus and volumetric properties of chondrocytes isolated from normal and osteoarthritic human cartilage. J Biomech. 1999; 32(2):119-27. DOI: 10.1016/s0021-9290(98)00166-3. View

12.

Henderson E, Haydon P, Sakaguchi D . Actin filament dynamics in living glial cells imaged by atomic force microscopy. Science. 1992; 257(5078):1944-6. DOI: 10.1126/science.1411511. View

13.

Heinz W, Antonik M, DCosta N, Nageswaran S, Schoenenberger C, Hoh J . Relative microelastic mapping of living cells by atomic force microscopy. Biophys J. 1998; 74(3):1564-78. PMC: 1299502. DOI: 10.1016/S0006-3495(98)77868-3. View

14.

Lekka M, Laidler P, Gil D, Lekki J, Stachura Z, Hrynkiewicz A . Elasticity of normal and cancerous human bladder cells studied by scanning force microscopy. Eur Biophys J. 1999; 28(4):312-6. DOI: 10.1007/s002490050213. View

15.

Sipkema P, van der Linden P, Westerhof N, Yin F . Effect of cyclic axial stretch of rat arteries on endothelial cytoskeletal morphology and vascular reactivity. J Biomech. 2003; 36(5):653-9. DOI: 10.1016/s0021-9290(02)00443-8. View

16.

Costa K, Lee E, Holmes J . Creating alignment and anisotropy in engineered heart tissue: role of boundary conditions in a model three-dimensional culture system. Tissue Eng. 2003; 9(4):567-77. DOI: 10.1089/107632703768247278. View

17.

Rotsch C, Jacobson K, Radmacher M . Dimensional and mechanical dynamics of active and stable edges in motile fibroblasts investigated by using atomic force microscopy. Proc Natl Acad Sci U S A. 1999; 96(3):921-6. PMC: 15326. DOI: 10.1073/pnas.96.3.921. View

18.

Pourati J, Maniotis A, Spiegel D, Schaffer J, Butler J, Fredberg J . Is cytoskeletal tension a major determinant of cell deformability in adherent endothelial cells?. Am J Physiol. 1998; 274(5):C1283-9. DOI: 10.1152/ajpcell.1998.274.5.C1283. View

19.

Ingber D . Tensegrity: the architectural basis of cellular mechanotransduction. Annu Rev Physiol. 1997; 59:575-99. DOI: 10.1146/annurev.physiol.59.1.575. View

20.

Evans E, Yeung A . Apparent viscosity and cortical tension of blood granulocytes determined by micropipet aspiration. Biophys J. 1989; 56(1):151-60. PMC: 1280460. DOI: 10.1016/S0006-3495(89)82660-8. View