Treatment with Cationic Liposome-DNA Complexes (CLDCs) Protects Mice from Lethal Western Equine Encephalitis Virus (WEEV) Challenge

Overview

Journal Antiviral Res

Publisher Elsevier

Date 2010 May 11

PMID 20452378

Citations 12

Authors

Christopher H Logue

Aaron T Phillips

Eric C Mossel

Jeremy P Ledermann

Thomas Welte

Steve W Dow

Ken E Olson

Ann M Powers

Affiliations

Soon will be listed here.

Abstract

Having recently characterized a CD-1 outbred mouse model of pathogenesis for Western equine encephalitis virus, we examined the possible protective effects of cationic liposome-DNA complexes (CLDCs) against encephalitic arboviral infection. In this investigation, mice were pre-treated, co-treated, or post-treated with CLDC then challenged with a subcutaneous or aerosol dose of the highly virulent WEEV-McMillan strain, lethal in mice 4-5 days after inoculation. Pre-treatment with CLDCs provided a significant protective effect in mice, which was reflected in significantly increased survival rates. Further, in some instances a therapeutic effect of CLDC administration up to 12h after WEEV challenge was observed. Mice treated with CLDC had significantly increased serum IFN-gamma, TNF-alpha, and IL-12, suggesting a strong Th1-biased antiviral activation of the innate immune system. In virus-infected animals, large increases in production of IFN-gamma, TNF-alpha, MCP-1, IL-12, and IL-10 in the brain were observed by 72h after infection, consistent with neuroinvasion and viral replication in the CNS. These results indicate that strong non-specific activation of innate immunity with an immune therapeutic such as CLDC is capable of eliciting significant protective immunity against a rapidly lethal strain of WEEV and suggest a possible prophylactic option for exposed individuals.

Citing Articles

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Wheat W, Chow L, Betlach A, Pieters M, Kurihara J, Dow C Animals (Basel). 2023; 13(10).

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Nanoparticle ocular immunotherapy for herpesvirus surface eye infections evaluated in cat infection model.

Lappin M, Wotman K, Chow L, Williams M, Hawley J, Dow S PLoS One. 2023; 18(1):e0279462.

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Immunopathogenesis of alphaviruses.

Baxter V, Heise M Adv Virus Res. 2020; 107:315-382.

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Non-specific protection from respiratory tract infections in cattle generated by intranasal administration of an innate immune stimulant.

Wheat W, Chow L, Rozo V, Herman J, Brooks K, Colbath A PLoS One. 2020; 15(6):e0235422.

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Local immune and microbiological responses to mucosal administration of a Liposome-TLR agonist immunotherapeutic in dogs.

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