Global Analysis of the Rat and Human Platelet Proteome - the Molecular Blueprint for Illustrating Multi-functional Platelets and Cross-species Function Evolution

Overview

Journal Proteomics

Date 2010 May 6

PMID 20443191

Citations 12

Authors

Yanbao Yu

Taohua Leng

Dong Yun

Na Liu

Jun Yao

Ying Dai

Pengyuan Yang

Xian Chen

Affiliations

Soon will be listed here.

Abstract

Emerging evidences indicate that blood platelets function in multiple biological processes including immune response, bone metastasis and liver regeneration in addition to their known roles in hemostasis and thrombosis. Global elucidation of platelet proteome will provide the molecular base of these platelet functions. Here, we set up a high-throughput platform for maximum exploration of the rat/human platelet proteome using integrated proteomic technologies, and then applied to identify the largest number of the proteins expressed in both rat and human platelets. After stringent statistical filtration, a total of 837 unique proteins matched with at least two unique peptides were precisely identified, making it the first comprehensive protein database so far for rat platelets. Meanwhile, quantitative analyses of the thrombin-stimulated platelets offered great insights into the biological functions of platelet proteins and therefore confirmed our global profiling data. A comparative proteomic analysis between rat and human platelets was also conducted, which revealed not only a significant similarity, but also an across-species evolutionary link that the orthologous proteins representing "core proteome", and the "evolutionary proteome" is actually a relatively static proteome.

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