Fluorescent Metal Nanoshell Probe to Detect Single MiRNA in Lung Cancer Cell

Overview

Journal Anal Chem

Specialty Chemistry

Date 2010 May 4

PMID 20433154

Citations 19

Authors

Jian Zhang

Yi Fu

Yuping Mei

Feng Jiang

Joseph R Lakowicz

Affiliations

Soon will be listed here.

Abstract

In this study, fluorescent metal nanoshells were synthesized as a molecular imaging agent to detect single microRNA (miRNA) molecules in the cells positive to lung cancer. These metal nanoshells were composed of silica spheres with encapsulated Ru(bpy)(3)(2+) complexes as cores and thin silver layers as shells. Compared with the silica spheres in the absence of metal, the metal nanoshells displayed an enhanced emission intensity, shortened lifetime, and extended photostability. The single-stranded probe oligonucleotides were covalently bound on the metal nanoshells to hybridize with the target miRNA-486 molecules in the cells. It was shown that with stronger emission intensity and longer lifetime, the conjugated metal nanoshells were isolated distinctly from the cellular autofluorescence on the cell images. These emission spots on the cell images were counted accurately and analyzed with a pool of cells representing the miRNA-486 expression levels in the cells. The results may reflect a genomic signal change and provide a reference to lung cancer early diagnosis as well as other diseases.

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References

Rosi N, Mirkin C . Nanostructures in biodiagnostics. Chem Rev. 2005; 105(4):1547-62. DOI: 10.1021/cr030067f. View

Templeton A, Wuelfing W, Murray R . Monolayer-protected cluster molecules. Acc Chem Res. 2000; 33(1):27-36. DOI: 10.1021/ar9602664. View

Zhang J, Fu Y, Liang D, Nowaczyk K, Zhao R, Lakowicz J . Single-cell fluorescence imaging using metal plasmon-coupled probe 2: single-molecule counting on lifetime image. Nano Lett. 2008; 8(4):1179-86. PMC: 2739996. DOI: 10.1021/nl080093z. View

Alvarez-Puebla R, Ross D, Nazri G, Aroca R . Surface-enhanced Raman scattering on nanoshells with tunable surface plasmon resonance. Langmuir. 2005; 21(23):10504-8. DOI: 10.1021/la051645l. View

Hubert C, Rumyantseva A, Lerondel G, Grand J, Kostcheev S, Billot L . Near-field photochemical imaging of noble metal nanostructures. Nano Lett. 2005; 5(4):615-9. DOI: 10.1021/nl047956i. View

Chatterjee S, Zetter B . Cancer biomarkers: knowing the present and predicting the future. Future Oncol. 2006; 1(1):37-50. DOI: 10.1517/14796694.1.1.37. View

Jiang Z, Li R, Todd N, Stass S, Jiang F . Detecting genomic aberrations by fluorescence in situ hybridization with quantum dots-labeled probes. J Nanosci Nanotechnol. 2008; 7(12):4254-9. DOI: 10.1166/jnn.2007.920. View

Lakowicz J . Radiative decay engineering: biophysical and biomedical applications. Anal Biochem. 2001; 298(1):1-24. PMC: 6901132. DOI: 10.1006/abio.2001.5377. View

Thunnissen F . Sputum examination for early detection of lung cancer. J Clin Pathol. 2003; 56(11):805-10. PMC: 1770101. DOI: 10.1136/jcp.56.11.805. View

10.

Lal S, Clare S, Halas N . Nanoshell-enabled photothermal cancer therapy: impending clinical impact. Acc Chem Res. 2008; 41(12):1842-51. DOI: 10.1021/ar800150g. View

11.

Varella-Garcia M, Kittelson J, Schulte A, Vu K, Wolf H, Zeng C . Multi-target interphase fluorescence in situ hybridization assay increases sensitivity of sputum cytology as a predictor of lung cancer. Cancer Detect Prev. 2004; 28(4):244-51. DOI: 10.1016/j.cdp.2004.04.007. View

12.

Zhang J, Fu Y, Lakowicz J . Luminescent Silica Core / Silver Shell Encapsulated with Eu(III) Complex. J Phys Chem C Nanomater Interfaces. 2010; 113(45):19404-19410. PMC: 2877519. DOI: 10.1021/jp906742q. View

13.

Kaur H, Babu B, Maiti S . Perspectives on chemistry and therapeutic applications of Locked Nucleic Acid (LNA). Chem Rev. 2007; 107(11):4672-97. DOI: 10.1021/cr050266u. View

14.

Zhang J, Fu Y, Chowdhury M, Lakowicz J . Enhanced Förster Resonance Energy Transfer on Single Metal Particle. 2. Dependence on Donor-Acceptor Separation Distance, Particle Size, and Distance from Metal Surface. J Phys Chem C Nanomater Interfaces. 2009; 111(32):11784-11792. PMC: 2772812. DOI: 10.1021/jp067887r. View

15.

Thomas J, Hergenrother P . Targeting RNA with small molecules. Chem Rev. 2008; 108(4):1171-224. DOI: 10.1021/cr0681546. View

16.

Colby T, Wistuba I, Gazdar A . Precursors to pulmonary neoplasia. Adv Anat Pathol. 1998; 5(4):205-15. DOI: 10.1097/00125480-199807000-00001. View

17.

Zhang J, Fu Y, Lakowicz J . Emission Behavior of Fluorescently Labeled Silver Nanoshell: Enhanced Self-Quenching by Metal Nanostructure. J Phys Chem C Nanomater Interfaces. 2009; 111(5):1955-1961. PMC: 2763911. DOI: 10.1021/jp063996u. View

18.

Yanaihara N, Caplen N, Bowman E, Seike M, Kumamoto K, Yi M . Unique microRNA molecular profiles in lung cancer diagnosis and prognosis. Cancer Cell. 2006; 9(3):189-98. DOI: 10.1016/j.ccr.2006.01.025. View

19.

Seike M, Goto A, Okano T, Bowman E, Schetter A, Horikawa I . MiR-21 is an EGFR-regulated anti-apoptotic factor in lung cancer in never-smokers. Proc Natl Acad Sci U S A. 2009; 106(29):12085-90. PMC: 2715493. DOI: 10.1073/pnas.0905234106. View

20.

Xie Y, Todd N, Liu Z, Zhan M, Fang H, Peng H . Altered miRNA expression in sputum for diagnosis of non-small cell lung cancer. Lung Cancer. 2009; 67(2):170-6. PMC: 2846426. DOI: 10.1016/j.lungcan.2009.04.004. View