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BsiY I, a Novel Thermophilic Restriction Endonuclease That Recognizes 5' CCNNNNNNNGG 3' and the Discovery of a Wrongly Sequenced Site in PACYC177

Overview
Journal Nucleic Acids Res
Publisher Oxford University Press
Specialty Biochemistry
Date 1991 May 11
PMID 2041772
Citations 5
Authors
Y K Mok
D R Clark
K M Kam
P C Shaw
Affiliations
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Abstract

A new type II restriction endonuclease designated BsiY I has been purified from a thermophilic soil Bacillus stearothermophilus strain. This enzyme recognizes and cleaves the highly degenerate sequence 5' CCNNNNN!NNGG 3'. During the identification of the recognition sequence of BsiY I, we discovered that there should be five G nucleotides instead of four at position 1227-1230 of the plasmid pACYC177.

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References
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