Identification and Molecular Characterization of Begomovirus and Associated Satellite DNA Molecules Infecting Cyamopsis Tetragonoloba

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 2010 Apr 21

PMID 20405195

Citations 10

Authors

J Kumar

A Kumar

J K Roy

R Tuli

J A Khan

Affiliations

Soon will be listed here.

Abstract

Monopartite begomoviruses comprise DNA-A as the main genome and associated satellite DNAs. Viral DNA extracted from guar (Cyamopsis tetragonoloba) showing leaf curl symptoms exhibited positive amplification of coat protein (CP) gene of DNA-A component, suggesting the presence of begomovirus. Full length DNA-A was amplified by primer pair re-designed from CP gene nucleotide sequence. The associated alphasatellite and betasatellite DNA molecules were amplified and sequenced, confirming the presence of monopartite begomovirus. Sequence comparisons showed 89% identity with other begomoviruses. The Neighbor-Joining tree based on full length DNA-A nucleotide sequence showed that the guar infecting begomovirus clustered separately from other known begomoviruses. The betasatellite shared a high (96%) nucleotide identity to Cotton leaf curl Multan betasatellites. The alphasatellite showed 91% nucleotide identity to alphasatellite associated with begomovirus infecting Okra. Recombination analyses showed three recombinant fragments in DNA-A, two in betasatellite, and four in alphasatellite. The results suggest that the begomovirus identified in this study was a new recombinant virus. Its name was proposed as Cyamopsis tetragonoloba leaf curl virus (CyTLCuV).

Citing Articles

Begomovirus and DNA-satellites association with mosaic and leaf curl disease of and : the new hosts for chilli leaf curl virus.

Venkataravanappa V, Ashwathappa K, Hiremath S, Manjunatha L, Shankarappa K, Krishna Reddy M Virusdisease. 2023; 34(4):504-513.

PMID: 38046062 PMC: 10686937. DOI: 10.1007/s13337-023-00850-x.

Sentinel plot surveillance of cotton leaf curl disease in Pakistan- a case study at the cultivated cotton-wild host plant interface.

Iqbal M, Zia-Ur-Rehman M, Ilyas M, Hameed U, Herrmann H, Chingandu N Virus Res. 2023; 333:199144.

PMID: 37271420 PMC: 10352719. DOI: 10.1016/j.virusres.2023.199144.

Molecular characterization of novel bipartite begomovirus associated with enation leaf disease of Garden croton ( L.).

Venkataravanappa V, Vinaykumar H, Hiremath S, Mantesh M, Shankarappa K, Basha C Virusdisease. 2022; 33(2):194-207.

PMID: 35991698 PMC: 9381679. DOI: 10.1007/s13337-022-00772-0.

Characterization of Tomato leaf curl New Delhi virus associated with leaf curl and yellowing disease of Watermelon and development of LAMP assay for its detection.

Venkataravanappa V, Ashwathappa K, Lakshminarayana Reddy C, Shankarappa K, Krishna Reddy M 3 Biotech. 2020; 10(6):282.

PMID: 32550101 PMC: 7266909. DOI: 10.1007/s13205-020-02245-x.

Association of Tomato Leaf Curl New Delhi Virus, Betasatellite, and Alphasatellite with Mosaic Disease of Spine Gourd ( Willd) in India.

Venkataravanappa V, Lakshminarayana Reddy C, Shankarappa K, Krishna Reddy M Iran J Biotechnol. 2019; 17(1):e2134.

PMID: 31457044 PMC: 6697862. DOI: 10.21859/ijb.2134.

References

Briddon R, Brown J, Moriones E, Stanley J, Zerbini M, Zhou X . Recommendations for the classification and nomenclature of the DNA-beta satellites of begomoviruses. Arch Virol. 2008; 153(4):763-81. DOI: 10.1007/s00705-007-0013-6. View

Rybicki E . A phylogenetic and evolutionary justification for three genera of Geminiviridae. Arch Virol. 1994; 139(1-2):49-77. DOI: 10.1007/BF01309454. View

Zhou X, Xie Y, Zhang Z, Qi Y, Wu J . Molecular characterization of a novel defective DNA isolated from tobacco tissues infected with tobacco leaf curl virus. Acta Virol. 2001; 45(1):45-50. View

Pita J, Fondong V, Sangare A, Otim-Nape G, Ogwal S, Fauquet C . Recombination, pseudorecombination and synergism of geminiviruses are determinant keys to the epidemic of severe cassava mosaic disease in Uganda. J Gen Virol. 2001; 82(Pt 3):655-665. DOI: 10.1099/0022-1317-82-3-655. View

Briddon R, Mansoor S, Bedford I, Pinner M, Saunders K, Stanley J . Identification of dna components required for induction of cotton leaf curl disease. Virology. 2001; 285(2):234-43. DOI: 10.1006/viro.2001.0949. View

Briddon R, Bull S, Amin I, Idris A, Mansoor S, Bedford I . Diversity of DNA beta, a satellite molecule associated with some monopartite begomoviruses. Virology. 2003; 312(1):106-21. DOI: 10.1016/s0042-6822(03)00200-9. View

Jose J, Usha R . Bhendi yellow vein mosaic disease in India is caused by association of a DNA Beta satellite with a begomovirus. Virology. 2003; 305(2):310-7. DOI: 10.1006/viro.2002.1768. View

Noris E, Hidalgo E, Accotto G, Moriones E . High similarity among the tomato yellow leaf curl virus isolates from the west Mediterranean basin: the nucleotide sequence of an infectious clone from Spain. Arch Virol. 1994; 135(1-2):165-70. DOI: 10.1007/BF01309774. View

Idris A, Brown J . Molecular analysis of Cotton leaf curl virus-Sudan reveals an evolutionary history of recombination. Virus Genes. 2002; 24(3):249-56. DOI: 10.1023/a:1015380600089. View

10.

Saunders K, Bedford I, Stanley J . Pathogenicity of a natural recombinant associated with ageratum yellow vein disease: implications for geminivirus evolution and disease aetiology. Virology. 2001; 282(1):38-47. DOI: 10.1006/viro.2000.0832. View

11.

Dry I, Rigden J, Krake L, Mullineaux P, Rezaian M . Nucleotide sequence and genome organization of tomato leaf curl geminivirus. J Gen Virol. 1993; 74 ( Pt 1):147-51. DOI: 10.1099/0022-1317-74-1-147. View

12.

Saunders K, Stanley J . A nanovirus-like DNA component associated with yellow vein disease of Ageratum conyzoides: evidence for interfamilial recombination between plant DNA viruses. Virology. 1999; 264(1):142-52. DOI: 10.1006/viro.1999.9948. View

13.

Monci F, Sanchez-Campos S, Navas-Castillo J, Moriones E . A natural recombinant between the geminiviruses Tomato yellow leaf curl Sardinia virus and Tomato yellow leaf curl virus exhibits a novel pathogenic phenotype and is becoming prevalent in Spanish populations. Virology. 2002; 303(2):317-26. DOI: 10.1006/viro.2002.1633. View

14.

Idris A, Brown J . Cotton leaf crumple virus Is a Distinct Western Hemisphere Begomovirus Species with Complex Evolutionary Relationships Indicative of Recombination and Reassortment. Phytopathology. 2008; 94(10):1068-74. DOI: 10.1094/PHYTO.2004.94.10.1068. View

15.

Tan P, Wong S, Wu M, Bedford I, Saunders K, Stanley J . Genome organization of ageratum yellow vein virus, a monopartite whitefly-transmitted geminivirus isolated from a common weed. J Gen Virol. 1995; 76 ( Pt 12):2915-22. DOI: 10.1099/0022-1317-76-12-2915. View

16.

Padidam M, Sawyer S, Fauquet C . Possible emergence of new geminiviruses by frequent recombination. Virology. 1999; 265(2):218-25. DOI: 10.1006/viro.1999.0056. View

17.

Navot N, Pichersky E, Zeidan M, Zamir D, Czosnek H . Tomato yellow leaf curl virus: a whitefly-transmitted geminivirus with a single genomic component. Virology. 1991; 185(1):151-61. DOI: 10.1016/0042-6822(91)90763-2. View

18.

Saeed M, Zafar Y, Randles J, Rezaian M . A monopartite begomovirus-associated DNA beta satellite substitutes for the DNA B of a bipartite begomovirus to permit systemic infection. J Gen Virol. 2007; 88(Pt 10):2881-2889. DOI: 10.1099/vir.0.83049-0. View

19.

Fauquet C, Briddon R, Brown J, Moriones E, Stanley J, Zerbini M . Geminivirus strain demarcation and nomenclature. Arch Virol. 2008; 153(4):783-821. DOI: 10.1007/s00705-008-0037-6. View

20.

Mayo M, Pringle C . Virus taxonomy--1997. J Gen Virol. 1998; 79 ( Pt 4):649-57. DOI: 10.1099/0022-1317-79-4-649. View