A Novel Antibiotic Based Long-term Model of Ovine Smoke Inhalation Injury and Septic Shock

Overview

Journal Burns

Publisher Elsevier

Date 2010 Apr 17

PMID 20395054

Citations 3

Authors

Yoshimitsu Nakano

Marc O Maybauer

Dirk M Maybauer

Perenlei Enkhbaatar

Daniel L Traber

Affiliations

Soon will be listed here.

Abstract

We modified our established and clinically relevant ARDS model of smoke inhalation injury and septic shock by administration of combined antibiotics (AB) such as piperacillin and ciprofloxacin, to more closely mimic the clinical intensive care setting. Twenty-three sheep were subjected to the injury, and allocated to four groups for a 96 h study period: sham (n=5 non-injured); control (n=6: injured); AB6h (n=6: injured, antibiotics started 6 h post-injury); AB12h (n=6: injured, antibiotics started 12 h post-injury). All sham animals survived 96 h. Control, AB6h, AB12h groups reached criteria of septic shock within 12 h post-injury. All controls died within 36 h. Eighty three percent of AB6h and fifty percent of AB12h survived 96 h. Median survival times were significantly improved in the treated groups compared with the control group: 24 h in control vs. 80.5 h in AB6h, and 65 h in AB12h animals. Combined ciprofloxacin and piperacillin therapy was effective, reduced nitric oxide production and mortality, and will allow future long-term studies in this model.

Citing Articles

Optimal management of the critically ill: anaesthesia, monitoring, data capture, and point-of-care technological practices in ovine models of critical care.

Chemonges S, Shekar K, Tung J, Dunster K, Diab S, Platts D Biomed Res Int. 2014; 2014:468309.

PMID: 24783206 PMC: 3982457. DOI: 10.1155/2014/468309.

The selective vasopressin type 1a receptor agonist selepressin (FE 202158) blocks vascular leak in ovine severe sepsis*.

Maybauer M, Maybauer D, Enkhbaatar P, Laporte R, Wisniewska H, Traber L Crit Care Med. 2014; 42(7):e525-e533.

PMID: 24674922 PMC: 4346299. DOI: 10.1097/CCM.0000000000000300.

The peroxynitrite catalyst WW-85 improves microcirculation in ovine smoke inhalation injury and septic shock.

Maybauer D, Maybauer M, Szabo C, Westphal M, Traber L, Salzman A Burns. 2011; 37(5):842-50.

PMID: 21345593 PMC: 3138828. DOI: 10.1016/j.burns.2010.12.020.

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