Characterization of Dental Caries by LIF Spectroscopy with 404-nm Excitation

Overview

Journal Lasers Med Sci

Publisher Springer

Date 2010 Apr 16

PMID 20393770

Citations 2

Authors

Shiny Sara Thomas

J L Jayanthi

Narayanan Subhash

Joji Thomas

Rupananda J Mallia

G N Aparna

Affiliations

Soon will be listed here.

Abstract

The potential of laser-induced fluorescence (LIF) spectroscopy for the characterization of different stages of dental caries using 404-nm diode laser excitation was investigated. In vitro spectra from 16 sound, 10 noncavitated carious and 10 cavitated carious molar teeth were recorded on a miniature fibre-optic spectrometer. The areas under the receiver operating characteristics (ROC-AUC) were calculated and one-way analysis of variance (ANOVA) was performed. The LIF spectra of the carious teeth showed two peaks at 635 and 680 nm in addition to a broad band seen at 500 nm in sound teeth. The fluorescence intensity ratios, F500/F635 and F500/F680, in carious teeth were always lower than those in sound teeth. The ROC-AUC for discriminating between carious and sound teeth was 0.94, and for discriminating between noncavitated and cavitated carious teeth was 0.87. Statistically significant differences (p<0.001) were seen between sound, noncavitated carious and cavitated carious teeth. The results showed that LIF spectroscopy has the potential to be useful for characterizing different stages of caries in a clinical setting.

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References

Subhash N, Thomas S, Mallia R, Jose M . Tooth caries detection by curve fitting of laser-induced fluorescence emission: a comparative evaluation with reflectance spectroscopy. Lasers Surg Med. 2005; 37(4):320-8. DOI: 10.1002/lsm.20229. View

Ribeiro A, Rousseau C, Girkin J, Hall A, Strang R, Whitters C . A preliminary investigation of a spectroscopic technique for the diagnosis of natural caries lesions. J Dent. 2005; 33(1):73-8. DOI: 10.1016/j.jdent.2004.08.006. View

Burin C, Burin C, Loguercio A, Grande R, Reis A . Occlusal caries detection: a comparison of a laser fluorescence system and conventional methods. Pediatr Dent. 2005; 27(4):307-12. View

Hall A, Deschepper E, Ando M, Stookey G . In vitro studies of laser fluorescence for detection and quantification of mineral loss from dental caries. Adv Dent Res. 1998; 11(4):507-14. DOI: 10.1177/08959374970110041901. View

Metz C . Basic principles of ROC analysis. Semin Nucl Med. 1978; 8(4):283-98. DOI: 10.1016/s0001-2998(78)80014-2. View

Borisova E, Uzunov T, Avramov L . Laser-induced autofluorescence study of caries model in vitro. Lasers Med Sci. 2006; 21(1):34-41. DOI: 10.1007/s10103-005-0365-7. View

Borisova E, Uzunov T, Avramov L . Early differentiation between caries and tooth demineralization using laser-induced autofluorescence spectroscopy. Lasers Surg Med. 2004; 34(3):249-53. DOI: 10.1002/lsm.20009. View

Olmez A, Tuna D, Oznurhan F . Clinical evaluation of diagnodent in detection of occlusal caries in children. J Clin Pediatr Dent. 2006; 30(4):287-91. View

Shi X, Tranaeus S, Angmar-Mansson B . Comparison of QLF and DIAGNOdent for quantification of smooth surface caries. Caries Res. 2000; 35(1):21-6. DOI: 10.1159/000047426. View

10.

Sanchez-Figueras Jr A . Occlusal pit-and-fissure caries diagnosis: a problem no more. A science-based diagnostic approach using a laser-based fluorescence device. Compend Contin Educ Dent. 2003; 24(5 Suppl):3-11; quiz 19. View

11.

Angnes V, Angnes G, Batisttella M, Grande R, Loguercio A, Reis A . Clinical effectiveness of laser fluorescence, visual inspection and radiography in the detection of occlusal caries. Caries Res. 2005; 39(6):490-5. DOI: 10.1159/000088185. View

12.

Shi X, Welander U, Angmar-Mansson B . Occlusal caries detection with KaVo DIAGNOdent and radiography: an in vitro comparison. Caries Res. 2000; 34(2):151-8. DOI: 10.1159/000016583. View

13.

Ferreira Zandona A, Analoui M, Beiswanger B, Isaacs R, Kafrawy A, Eckert G . An in vitro comparison between laser fluorescence and visual examination for detection of demineralization in occlusal pits and fissures. Caries Res. 1998; 32(3):210-8. DOI: 10.1159/000016455. View

14.

Ando M, van der Veen M, Schemehorn B, Stookey G . Comparative study to quantify demineralized enamel in deciduous and permanent teeth using laser- and light-induced fluorescence techniques. Caries Res. 2002; 35(6):464-70. DOI: 10.1159/000047491. View

15.

Alfano R, Yao S . Human teeth with and without dental caries studied by visible luminescent spectroscopy. J Dent Res. 1981; 60(2):120-2. DOI: 10.1177/00220345810600020401. View

16.

Konig K, FLEMMING G, Hibst R . Laser-induced autofluorescence spectroscopy of dental caries. Cell Mol Biol (Noisy-le-grand). 1999; 44(8):1293-300. View

17.

Attrill D, Ashley P . Occlusal caries detection in primary teeth: a comparison of DIAGNOdent with conventional methods. Br Dent J. 2001; 190(8):440-3. DOI: 10.1038/sj.bdj.4800998. View

18.

Zezell D, Ribeiro A, Bachmann L, Gomes A, Rousseau C, Girkin J . Characterization of natural carious lesions by fluorescence spectroscopy at 405-nm excitation wavelength. J Biomed Opt. 2008; 12(6):064013. DOI: 10.1117/1.2821192. View