Histological Changes in the Uterus of the Hens After Embryonic Exposure to Bisphenol A and Diethylstilbestrol

Overview

Journal Protoplasma

Publisher Springer

Specialty Biology

Date 2010 Apr 16

PMID 20393758

Citations 7

Authors

Funda Yigit

Suzan Daglioglu

Affiliations

Soon will be listed here.

Abstract

Many employed chemicals in industries have estrogenic hormone effects on organisms, and these are called as environmental estrogens. Environmental estrogens have adverse effects on development and function of reproductive organs of the birds. Bisphenol A (BPA) is one of the best known environmental estrogens widely found in plastic products. In this study, we injected BPA and the synthetic estrogen diethylstilbestrol (DES) in ovo and then examined and compared the effects of those on the uteri (shell gland) of the adult hens by histological methods. Five groups have been designed in the current study. Only vehicle substance was given in ovo to the control group and BPA (67 or 134 μg/g egg) and DES (0.02 or 0.2 μg/g egg) were administered in the experimental groups. Tissue specimens were taken from uteri of hens at 21 weeks of age, prior to the laying period. Estrogen receptor alpha (ERα) was immunohistochemically stained. It was observed that the hatching proportion in BPA (67 μg and 134 μg/g) was lesser than the other groups (P<0.01). Uterine tubular glandular density and thickness of tunica mucosa were found to have reduced (P<0.01) in BPA (134 μg/g) and DES (0.2 μg/g) groups, in comparison with those of the control and the other experimental groups. Uterine gland epithelium revealed positive immunoreaction for ERα. These findings suggested that administration of BPA and DES at high doses affected embryonic development in a negative way, and this adverse effect was seen less in adult period.

Citing Articles

Toxicological Impact of Bisphenol A on Females' Reproductive System: Review Based on Experimental and Epidemiological Studies.

Chaichian S, Khodabandehloo F, Haghighi L, Govahi A, Mehdizadeh M, Ajdary M Reprod Sci. 2024; 31(7):1781-1799.

PMID: 38532232 DOI: 10.1007/s43032-024-01521-y.

Effects of Bisphenol A and Bisphenol F on Porcine Uterus Contractility.

Zygmuntowicz A, Markiewicz W, Grabowski T, Jaroszewski J J Vet Res. 2022; 66(2):257-265.

PMID: 35892107 PMC: 9281530. DOI: 10.2478/jvetres-2022-0031.

Histomorphometrical changes of oviduct during the long-term exposure of breeder hens to extra thyroxine.

Saemi F, Shahneh A, Zhandi M, Akhlaghi A, Khaksar Z, Dadpasand M Iran J Vet Res. 2018; 19(2):118-122.

PMID: 30046323 PMC: 6056141.

Evidence for bisphenol A-induced female infertility: a review (2007-2016).

Ziv-Gal A, Flaws J Fertil Steril. 2016; 106(4):827-56.

PMID: 27417731 PMC: 5026908. DOI: 10.1016/j.fertnstert.2016.06.027.

Early programing of uterine tissue by bisphenol A: Critical evaluation of evidence from animal exposure studies.

Suvorov A, Waxman D Reprod Toxicol. 2015; 57:59-72.

PMID: 26028543 PMC: 4550532. DOI: 10.1016/j.reprotox.2015.05.008.

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