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Rapid Diagnosis of Old World Leishmaniasis by High-resolution Melting Analysis of the 7SL RNA Gene

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2010 Apr 16
PMID 20392923
Citations 25
Authors
Abedelmajeed Nasereddin
Charles L Jaffe
Affiliations
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Abstract

High-resolution melt analysis PCR (HRM PCR) for diagnosis of Old World Leishmania was developed using the 7SL RNA gene. Cutaneous leishmaniasis samples were analyzed. Sensitivity and specificity of HRM PCR were significantly better (P < 0.001) than those of internal transcribed spacer 1 PCR and similar to those of kinetoplast DNA PCR.

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