Trichostatin A Inhibits Expression of Cathepsins in Experimental Osteoarthritis

Overview

Journal Rheumatol Int

Specialty Rheumatology

Date 2010 Apr 15

PMID 20390279

Citations 9

Authors

Wei-Ping Chen

Jia-Peng Bao

Jin-Li Tang

Peng-Fei Hu

Li-Dong Wu

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to investigate the effects of trichostatin A (TSA) on expression of cathepsins in cartilage in experimental osteoarthritis (OA). OA was induced in 18 rabbits by bilateral anterior cruciate ligament transection (ACLT). Four weeks after surgery, rabbits received intra-articular injection with TSA dissolved in the dimethylsulphoxide (DMSO) in the right knees and DMSO in the left knees once a week for 5 weeks. Rabbits were killed 7 days after the last injection. The knee joints were assessed by morphological and histological examination. Messenger RNA expression of cathepsins K, B, L, S and cystatin C was studied by real-time PCR. TSA inhibited the expression of cathepsins K, B, L, S and cystatin C accompanied with the less degradation in cartilage. The results suggest that TSA exhibits protective effects against cartilage degradation in rabbits with OA and the effects may be associated with the inhibition of cathepsins.

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