Low-level Ozone Exposure Induces Airways Inflammation and Modifies Cell Surface Phenotypes in Healthy Humans

Overview

Journal Inhal Toxicol

Publisher Informa Healthcare

Specialty Toxicology

Date 2010 Apr 14

PMID 20384440

Citations 31

Authors

Neil E Alexis

John C Lay

Milan Hazucha

Bradford Harris

Michelle L Hernandez

Philip A Bromberg

Howard Kehrl

David Diaz-Sanchez

Chong Kim

Robert B Devlin

David B Peden

Affiliations

Soon will be listed here.

Abstract

The effects of low-level ozone exposure (0.08 ppm) on pulmonary function in healthy young adults are well known; however, much less is known about the inflammatory and immunomodulatory effects of low-level ozone in the airways. Techniques such as induced sputum and flow cytometry make it possible to examine airways inflammatory responses and changes in immune cell surface phenotypes following low-level ozone exposure. The purpose of this study was to determine if exposure to 0.08 parts per million ozone for 6.6 h induces inflammation and modifies immune cell surface phenotypes in the airways of healthy adult subjects. Fifteen normal volunteers underwent an established 0.08 part per million ozone exposure protocol to characterize the effect of ozone on airways inflammation and immune cell surface phenotypes. Induced sputum and flow cytometry were used to assess these endpoints 24 h before and 18 h after exposure. The results showed that exposure to 0.08 ppm ozone for 6.6 h induced increased airway neutrophils, monocytes, and dendritic cells and modified the expression of CD14, HLA-DR, CD80, and CD86 on monocytes 18 h following exposure. Exposure to 0.08 parts per million ozone is associated with increased airways inflammation and promotion of antigen-presenting cell phenotypes 18 hours following exposure. These findings need to be replicated in a similar experiment that includes a control air exposure.

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