Optimization of Data Collection Taking Radiation Damage into Account

Overview

Journal Acta Crystallogr D Biol Crystallogr

Specialty Chemistry

Date 2010 Apr 13

PMID 20382994

Citations 60

Authors

Gleb P Bourenkov

Alexander N Popov

Affiliations

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Abstract

To take into account the effects of radiation damage, new algorithms for the optimization of data-collection strategies have been implemented in the software package BEST. The intensity variation related to radiation damage is approximated by log-linear functions of resolution and cumulative X-ray dose. Based on an accurate prediction of the basic characteristics of data yet to be collected, BEST establishes objective relationships between the accessible data completeness, resolution and signal-to-noise statistics that can be achieved in an experiment and designs an optimal plan for data collection.

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