Robust Enhancement of Neural Differentiation from Human ES and IPS Cells Regardless of Their Innate Difference in Differentiation Propensity

Overview

Journal Stem Cell Rev Rep

Publisher Springer

Specialty Cell Biology

Date 2010 Apr 9

PMID 20376579

Citations 117

Authors

Dae-Sung Kim

Jae Souk Lee

Joong Woo Leem

Yong Jun Huh

Ji Young Kim

Han-Soo Kim

In-Hyun Park

George Q Daley

Dong-Youn Hwang

Dong-Wook Kim

Affiliations

Soon will be listed here.

Abstract

Our analyses of three human induced pluripotent stem cell (hiPSC) and six human embryonic stem cell (hESC) lines showed marked variability in differentiation potential into specific lineages, which often hampers their differentiation into specific cell types or cell lineages of interest. Simultaneous inhibition of both Activin/Nodal and BMP pathways with small molecules, SB431542 and dorsomorphin (DM), respectively, promoted significant neural differentiation from all human pluripotent stem cell (hPSC) lines tested, regardless of their differentiation propensity. On the contrary, differentiation into other cell lineages and the number of undifferentiated cells were significantly reduced after differentiation by the dual inhibition. These results demonstrate that innate differentiation propensity of hPSCs could be overcome, at least in part, by modulation of intracellular signaling pathways, resulting in efficient generation of desirable cell types, such as neural cells.

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