Recombinant Aequorin As a Probe for Cytosolic Free Ca2+ in Escherichia Coli

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 1991 May 6

PMID 2037058

Citations 15

Authors

M R Knight

A K Campbell

S M Smith

A J Trewavas

Affiliations

Soon will be listed here.

Abstract

We describe a novel and simple method for the measurement of bacterial cytosolic free calcium ([Ca2+]i) using recombinant aequorin reconstituted within live bacterial cells. Using this method we have measured the effects of external calcium, complement, phagocytosis and antibiotics on the [Ca2+]i of Escherichia coli. In principle this method should be applicable to any genetically transformable organism and should suffer fewer problems than fluorescent dyes for subcellular calcium measurement.

Citing Articles

Methods for the detection of intracellular calcium in filamentous fungi.

Rasaily M, Ngiimei D S, Thaosen R, Gupta S, Deka S, Tamuli R MethodsX. 2024; 12:102570.

PMID: 38322134 PMC: 10844858. DOI: 10.1016/j.mex.2024.102570.

Unveiling the Secrets of Calcium-Dependent Proteins in Plant Growth-Promoting Rhizobacteria: An Abundance of Discoveries Awaits.

Agaras B, Grossi C, Ulloa R Plants (Basel). 2023; 12(19).

PMID: 37836138 PMC: 10574481. DOI: 10.3390/plants12193398.

Protection of the DNA from Selected Species of Five Kingdoms in Nature by Ba(II), Sr(II), and Ca(II) Silica-Carbonates: Implications about Biogenicity and Evolving from Prebiotic Chemistry to Biological Chemistry.

Cuellar-Cruz M, Islas S, Ramirez-Ramirez N, Pedraza-Reyes M, Moreno A ACS Omega. 2022; 7(42):37410-37426.

PMID: 36312347 PMC: 9609056. DOI: 10.1021/acsomega.2c04170.

Plant Immune Memory in Systemic Tissue Does Not Involve Changes in Rapid Calcium Signaling.

Eichstadt B, Lederer S, Trempel F, Jiang X, Guerra T, Waadt R Front Plant Sci. 2021; 12:798230.

PMID: 34970294 PMC: 8712724. DOI: 10.3389/fpls.2021.798230.

Calcium Regulation of Bacterial Virulence.

King M, Kayastha B, Franklin M, Patrauchan M Adv Exp Med Biol. 2019; 1131:827-855.

PMID: 31646536 PMC: 7473484. DOI: 10.1007/978-3-030-12457-1_33.