Phase Behavior of Planar Supported Lipid Membranes Composed of Cholesterol and 1,2-Distearoyl-sn-Glycerol-3-Phosphocholine Examined by Sum-Frequency Vibrational Spectroscopy

Overview

Journal Vib Spectrosc

Publisher Elsevier

Date 2010 Apr 3

PMID 20361007

Citations 2

Authors

Jin Liu

John C Conboy

Affiliations

Soon will be listed here.

Abstract

The influence of cholesterol (CHO) on the phase behavior of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) planar supported lipid bilayers (PSLBs) was investigated by sum-frequency vibrational spectroscopy (SFVS). The intrinsic symmetry constraints of SFVS were exploited to measure the asymmetric distribution of phase segregated phospholipid domains in the proximal and distal layers of DSPC + CHO binary mixtures as a function of CHO content and temperature. The SFVS results suggest that cholesterol significantly affects the phase segregation and domain distribution in PSLBs of DSPC in a concentration dependent manner, similar to that found in bulk suspensions. The SFVS spectroscopic measurements of phase segregation and structure change in the binary mixture indicate that membrane asymmetry must be present in order for the changes in SFVS signal to be observed. These results therefore provide important evidence for the delocalization and segregation of different phase domain structures in PSLBs due to the interaction of cholesterol and phospholipids.

Citing Articles

Protein-phospholipid interactions in nonclassical protein secretion: problem and methods of study.

Prudovsky I, Kumar T, Sterling S, Neivandt D Int J Mol Sci. 2013; 14(2):3734-72.

PMID: 23396106 PMC: 3588068. DOI: 10.3390/ijms14023734.

A simplified sum-frequency vibrational imaging setup used for imaging lipid bilayer arrays.

Smith K, Conboy J Anal Chem. 2012; 84(19):8122-6.

PMID: 22947074 PMC: 3523694. DOI: 10.1021/ac301290e.

References

Huang T, Lee C, Gupta S, Blume A, Griffin R . A 13C and 2H nuclear magnetic resonance study of phosphatidylcholine/cholesterol interactions: characterization of liquid-gel phases. Biochemistry. 1993; 32(48):13277-87. DOI: 10.1021/bi00211a041. View

Mendelsohn R, Davies M, Brauner J, SCHUSTER H, Dluhy R . Quantitative determination of conformational disorder in the acyl chains of phospholipid bilayers by infrared spectroscopy. Biochemistry. 1989; 28(22):8934-9. DOI: 10.1021/bi00448a037. View

Veatch S, Polozov I, Gawrisch K, Keller S . Liquid domains in vesicles investigated by NMR and fluorescence microscopy. Biophys J. 2004; 86(5):2910-22. PMC: 1304159. DOI: 10.1016/S0006-3495(04)74342-8. View

Demel R, de Kruyff B . The function of sterols in membranes. Biochim Biophys Acta. 1976; 457(2):109-32. DOI: 10.1016/0304-4157(76)90008-3. View

Liu J, Conboy J . Structure of a gel phase lipid bilayer prepared by the Langmuir-Blodgett/Langmuir-Schaefer method characterized by sum-frequency vibrational spectroscopy. Langmuir. 2005; 21(20):9091-7. DOI: 10.1021/la051500e. View

Liu J, Conboy J . Phase transition of a single lipid bilayer measured by sum-frequency vibrational spectroscopy. J Am Chem Soc. 2004; 126(29):8894-5. DOI: 10.1021/ja031570c. View

Lange Y, Dolde J, Steck T . The rate of transmembrane movement of cholesterol in the human erythrocyte. J Biol Chem. 1981; 256(11):5321-3. View

Mabrey S, Mateo P, STURTEVANT J . High-sensitivity scanning calorimetric study of mixtures of cholesterol with dimyristoyl- and dipalmitoylphosphatidylcholines. Biochemistry. 1978; 17(12):2464-8. DOI: 10.1021/bi00605a034. View

McMullen T, LEWIS R, McElhaney R . Differential scanning calorimetric and Fourier transform infrared spectroscopic studies of the effects of cholesterol on the thermotropic phase behavior and organization of a homologous series of linear saturated phosphatidylserine bilayer membranes. Biophys J. 2000; 79(4):2056-65. PMC: 1301095. DOI: 10.1016/S0006-3495(00)76453-8. View

10.

Ipsen J, Karlstrom G, Mouritsen O, Wennerstrom H, Zuckermann M . Phase equilibria in the phosphatidylcholine-cholesterol system. Biochim Biophys Acta. 1987; 905(1):162-72. DOI: 10.1016/0005-2736(87)90020-4. View

11.

Reinl H, Brumm T, Bayerl T . Changes of the physical properties of the liquid-ordered phase with temperature in binary mixtures of DPPC with cholesterol: A H-NMR, FT-IR, DSC, and neutron scattering study. Biophys J. 2009; 61(4):1025-35. PMC: 1260362. DOI: 10.1016/S0006-3495(92)81910-0. View

12.

Loura L, Fedorov A, Prieto M . Fluid-fluid membrane microheterogeneity: a fluorescence resonance energy transfer study. Biophys J. 2001; 80(2):776-88. PMC: 1301276. DOI: 10.1016/S0006-3495(01)76057-2. View

13.

Binder W, Barragan V, Menger F . Domains and rafts in lipid membranes. Angew Chem Int Ed Engl. 2003; 42(47):5802-27. DOI: 10.1002/anie.200300586. View

14.

Recktenwald D, McConnell H . Phase equilibria in binary mixtures of phosphatidylcholine and cholesterol. Biochemistry. 1981; 20(15):4505-10. DOI: 10.1021/bi00518a042. View

15.

Borden M, Martinez G, Ricker J, Tsvetkova N, Longo M, Gillies R . Lateral phase separation in lipid-coated microbubbles. Langmuir. 2006; 22(9):4291-7. DOI: 10.1021/la052841v. View

16.

Anderson T, McConnell H . Condensed complexes and the calorimetry of cholesterol-phospholipid bilayers. Biophys J. 2001; 81(5):2774-85. PMC: 1301744. DOI: 10.1016/S0006-3495(01)75920-6. View

17.

Kato T . Self-assembly of phase-segregated liquid crystal structures. Science. 2002; 295(5564):2414-8. DOI: 10.1126/science.1070967. View

18.

Gaber B, Peticolas W . On the quantitative interpretation of biomembrane structure by Raman spectroscopy. Biochim Biophys Acta. 1977; 465(2):260-74. DOI: 10.1016/0005-2736(77)90078-5. View

19.

Hu Y, Meleson K, Israelachvili J . Thermodynamic equilibrium of domains in a two-component Langmuir monolayer. Biophys J. 2006; 91(2):444-53. PMC: 1483102. DOI: 10.1529/biophysj.106.081000. View

20.

London E, Brown D . Insolubility of lipids in triton X-100: physical origin and relationship to sphingolipid/cholesterol membrane domains (rafts). Biochim Biophys Acta. 2000; 1508(1-2):182-95. DOI: 10.1016/s0304-4157(00)00007-1. View