Efficient Gene Delivery and Silencing of Mouse and Human Pancreatic Islets

Overview

Journal BMC Biotechnol

Publisher Biomed Central

Specialty Biotechnology

Date 2010 Apr 1

PMID 20353585

Citations 9

Authors

Bruno Lefebvre

Brigitte Vandewalle

Justine Longue

Ericka Moerman

Bruno Lukowiak

Valery Gmyr

Kathrin Maedler

Julie Kerr-Conte

Francois Pattou

Affiliations

Soon will be listed here.

Abstract

Background: In view of the importance of beta cells in glucose homeostasis and the profound repercussions of beta cell pathology on human health, the acquisition of tools to study pancreatic islet function is essential for the design of alternative novel therapies for diabetes. One promising approach toward this goal involves the modification of gene expression profile of beta cells.

Results: This study describes a new method of gene and siRNA delivery into human pancreatic islets by microporation technology. We demonstrated that mild islet distention with accutase greatly enhanced the transfection efficiency without compromising in vitro function (secretion, apoptosis and viability). As an example, the recently identified gene involved in type 2 diabetes, ZnT8, can be over-expressed or silenced by RNA interference using this technology. Microporation can also be used on rodent islets.

Conclusions: Taken together, our results demonstrate that microporation technology can be used to modify gene expression in whole rodent and human islets without altering their in vitro function and will be key to the elucidation of the factors responsible for proper islet function.

Citing Articles

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Co-opting regulation bypass repair as a gene-correction strategy for monogenic diseases.

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Pre-Microporation Improves Outcome of Pancreatic Islet Labelling for Optical and F MR Imaging.

Herynek V, Galisova A, Srinivas M, van Dinther E, Kosinova L, Ruzicka J Biol Proced Online. 2017; 19:6.

PMID: 28674481 PMC: 5488379. DOI: 10.1186/s12575-017-0055-4.

A Simple High Efficiency Intra-Islet Transduction Protocol Using Lentiviral Vectors.

Jimenez-Moreno C, Herrera-Gomez I, Lopez-Noriega L, Lorenzo P, Cobo-Vuilleumier N, Fuente-Martin E Curr Gene Ther. 2015; 15(4):436-46.

PMID: 26122098 PMC: 5411998. DOI: 10.2174/1566523215666150630121557.

Inhibition of the glucose transporter SGLT2 with dapagliflozin in pancreatic alpha cells triggers glucagon secretion.

Bonner C, Kerr-Conte J, Gmyr V, Queniat G, Moerman E, Thevenet J Nat Med. 2015; 21(5):512-7.

PMID: 25894829 DOI: 10.1038/nm.3828.

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