Sugarcane DIRIGENT and O-methyltransferase Promoters Confer Stem-regulated Gene Expression in Diverse Monocots

Overview

Journal Planta

Specialty Biology

Date 2010 Mar 31

PMID 20352262

Citations 27

Authors

Mona B Damaj

Siva P Kumpatla

Chandrakanth Emani

Phillip D Beremand

Avutu S Reddy

Keerti S Rathore

Marco T Buenrostro-Nava

Ian S Curtis

Terry L Thomas

T Erik Mirkov

Affiliations

Soon will be listed here.

Abstract

Transcription profiling analysis identified Saccharum hybrid DIRIGENT (SHDIR16) and Omicron-Methyltransferase (SHOMT), putative defense and fiber biosynthesis-related genes that are highly expressed in the stem of sugarcane, a major sucrose accumulator and biomass producer. Promoters (Pro) of these genes were isolated and fused to the beta-glucuronidase (GUS) reporter gene. Transient and stable transgene expression analyses showed that both Pro( DIR16 ):GUS and Pro( OMT ):GUS retain the expression characteristics of their respective endogenous genes in sugarcane and function in orthologous monocot species, including rice, maize and sorghum. Furthermore, both promoters conferred stem-regulated expression, which was further enhanced in the stem and induced in the leaf and root by salicylic acid, jasmonic acid and methyl jasmonate, key regulators of biotic and abiotic stresses. Pro( DIR16 ) and Pro( OMT ) will enable functional gene analysis in monocots, and will facilitate engineering monocots for improved carbon metabolism, enhanced stress tolerance and bioenergy production.

Citing Articles

CRISPR/Cas technology: fueling the future of Biofuel production with sugarcane.

Ghane A, Malhotra P, Sanghera G, Verma S, Jamwal N, Kashyap L Funct Integr Genomics. 2024; 24(6):205.

PMID: 39495322 DOI: 10.1007/s10142-024-01487-9.

Ectopic and transient expression of VvDIR4 gene in Arabidopsis and grapes enhances resistance to anthracnose via affecting hormone signaling pathways and lignin production.

Zhang Q, Luo N, Dai X, Lin J, Ahmad B, Chen Q BMC Genomics. 2024; 25(1):895.

PMID: 39342082 PMC: 11439227. DOI: 10.1186/s12864-024-10830-0.

Insights into the functional characterization of DIR proteins through genome-wide in silico and evolutionary studies: a systematic review.

Kapoor P, Rakhra G, Kumar V, Joshi R, Gupta M, Rakhra G Funct Integr Genomics. 2023; 23(2):166.

PMID: 37202648 DOI: 10.1007/s10142-023-01095-z.

Overexpression of Sugarcane Genes Enhances Drought Tolerance in .

Li X, Liu Z, Zhao H, Deng X, Su Y, Li R Int J Mol Sci. 2022; 23(10).

PMID: 35628151 PMC: 9141896. DOI: 10.3390/ijms23105340.

Association of gene expression with syringyl to guaiacyl ratio in sugarcane lignin.

Hodgson-Kratky K, Perlo V, Furtado A, Choudhary H, Gladden J, Simmons B Plant Mol Biol. 2021; 106(1-2):173-192.

PMID: 33738678 DOI: 10.1007/s11103-021-01136-w.

References

Rouster J, Leah R, Mundy J, Cameron-Mills V . Identification of a methyl jasmonate-responsive region in the promoter of a lipoxygenase 1 gene expressed in barley grain. Plant J. 1997; 11(3):513-23. DOI: 10.1046/j.1365-313x.1997.11030513.x. View

Yu S, Ko S, Hong C, Sun H, Hsing Y, Tong C . Global functional analyses of rice promoters by genomics approaches. Plant Mol Biol. 2007; 65(4):417-25. DOI: 10.1007/s11103-007-9232-1. View

Dwivedi U, Campbell W, Yu J, Datla R, Bugos R, Chiang V . Modification of lignin biosynthesis in transgenic Nicotiana through expression of an antisense O-methyltransferase gene from Populus. Plant Mol Biol. 1994; 26(1):61-71. DOI: 10.1007/BF00039520. View

Sattler S, Saathoff A, Haas E, Palmer N, Funnell-Harris D, Sarath G . A nonsense mutation in a cinnamyl alcohol dehydrogenase gene is responsible for the Sorghum brown midrib6 phenotype. Plant Physiol. 2009; 150(2):584-95. PMC: 2689950. DOI: 10.1104/pp.109.136408. View

Jefferson R, Kavanagh T, Bevan M . GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987; 6(13):3901-7. PMC: 553867. DOI: 10.1002/j.1460-2075.1987.tb02730.x. View

Hwang S, Lee I, Yie S, Hwang D . Identification of an OsPR10a promoter region responsive to salicylic acid. Planta. 2008; 227(5):1141-50. PMC: 2270913. DOI: 10.1007/s00425-007-0687-8. View

Fristensky B, Horovitz D, Hadwiger L . cDNA sequences for pea disease resistance response genes. Plant Mol Biol. 2013; 11(5):713-5. DOI: 10.1007/BF00017470. View

Altpeter F, Varshney A, Abderhalden O, Douchkov D, Sautter C, Kumlehn J . Stable expression of a defense-related gene in wheat epidermis under transcriptional control of a novel promoter confers pathogen resistance. Plant Mol Biol. 2005; 57(2):271-83. DOI: 10.1007/s11103-004-7564-7. View

Tomkins J, Yu Y, Miller-Smith H, Frisch D, Woo S, Wing R . A bacterial artificial chromosome library for sugarcane. Theor Appl Genet. 2012; 99(3-4):419-24. DOI: 10.1007/s001220051252. View

10.

Boerjan W, Ralph J, Baucher M . Lignin biosynthesis. Annu Rev Plant Biol. 2003; 54:519-46. DOI: 10.1146/annurev.arplant.54.031902.134938. View

11.

da Costa E Silva O, Klein L, Schmelzer E, Trezzini G, Hahlbrock K . BPF-1, a pathogen-induced DNA-binding protein involved in the plant defense response. Plant J. 1993; 4(1):125-35. DOI: 10.1046/j.1365-313x.1993.04010125.x. View

12.

Piquemal J, Chamayou S, Nadaud I, Beckert M, Barriere Y, Mila I . Down-regulation of caffeic acid o-methyltransferase in maize revisited using a transgenic approach. Plant Physiol. 2002; 130(4):1675-85. PMC: 166682. DOI: 10.1104/pp.012237. View

13.

Patzlaff A, Newman L, Dubos C, Whetten R, Smith C, McInnis S . Characterisation of Pt MYB1, an R2R3-MYB from pine xylem. Plant Mol Biol. 2004; 53(4):597-608. DOI: 10.1023/B:PLAN.0000019066.07933.d6. View

14.

Held B, Wang H, John I, Wurtele E, Colbert J . An mRNA putatively coding for an O-methyltransferase accumulates preferentially in maize roots and is located predominantly in the region of the endodermis. Plant Physiol. 1993; 102(3):1001-8. PMC: 158874. DOI: 10.1104/pp.102.3.1001. View

15.

Mudge S, Osabe K, Casu R, Bonnett G, Manners J, Birch R . Efficient silencing of reporter transgenes coupled to known functional promoters in sugarcane, a highly polyploid crop species. Planta. 2008; 229(3):549-58. DOI: 10.1007/s00425-008-0852-8. View

16.

Hatfield R, Vermerris W . Lignin formation in plants. The dilemma of linkage specificity. Plant Physiol. 2001; 126(4):1351-7. PMC: 1540132. DOI: 10.1104/pp.126.4.1351. View

17.

Tsai , Popko , Mielke , Hu , Podila , Chiang . Suppression of O-methyltransferase gene by homologous sense transgene in quaking aspen causes red-brown wood phenotypes . Plant Physiol. 1998; 117(1):101-12. PMC: 34993. DOI: 10.1104/pp.117.1.101. View

18.

Liu C, Deavours B, Richard S, Ferrer J, Blount J, Huhman D . Structural basis for dual functionality of isoflavonoid O-methyltransferases in the evolution of plant defense responses. Plant Cell. 2006; 18(12):3656-69. PMC: 1785397. DOI: 10.1105/tpc.106.041376. View

19.

Goujon T, Sibout R, Pollet B, Maba B, Nussaume L, Bechtold N . A new Arabidopsis thaliana mutant deficient in the expression of O-methyltransferase impacts lignins and sinapoyl esters. Plant Mol Biol. 2003; 51(6):973-89. DOI: 10.1023/a:1023022825098. View

20.

Wei H, Wang M, Moore P, Albert H . Comparative expression analysis of two sugarcane polyubiquitin promoters and flanking sequences in transgenic plants. J Plant Physiol. 2003; 160(10):1241-51. DOI: 10.1078/0176-1617-01086. View