Prp4 from Schizosaccharomyces Pombe, a Mutant Deficient in Pre-mRNA Splicing Isolated Using Genes Containing Artificial Introns

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1991 Apr 1

PMID 2034223

Citations 38

Authors

G H Rosenberg

S K Alahari

N F Kaufer

Affiliations

Soon will be listed here.

Abstract

We have generated a bank of temperature-sensitive (ts) Schizosaccharomyces pombe mutant strains. About 150 of these mutants were transformed with a ura4 gene containing an artificial intron. We screened these ts mutants for mutants deficient in splicing of the ura4 intron. With this approach three mutants were isolated which have a general defect in the splicing process. Two of these mutants fall into the prp1 complementation group and one defines a new complementation group, prp4.

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Clarke L, Cook A, Mathavarajah S, Bera A, Salsman J, Habib E FASEB J. 2021; 35(11):e22001.

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