The Elegans of Spindle Assembly

Overview

Journal Cell Mol Life Sci

Publisher Springer

Specialty Biology

Date 2010 Mar 27

PMID 20339898

Citations 25

Authors

Thomas Muller-Reichert

Garrett Greenan

Eileen OToole

Martin Srayko

Affiliations

Soon will be listed here.

Abstract

The Caenorhabditis elegans one-cell embryo is a powerful system in which to study microtubule organization because this large cell assembles both meiotic and mitotic spindles within the same cytoplasm over the course of 1 h in a stereotypical manner. The fertilized oocyte assembles two consecutive acentrosomal meiotic spindles that function to reduce the replicated maternal diploid set of chromosomes to a single-copy haploid set. The resulting maternal DNA then unites with the paternal DNA to form a zygotic diploid complement, around which a centrosome-based mitotic spindle forms. The early C. elegans embryo is amenable to live-cell imaging and electron tomography, permitting a detailed structural comparison of the meiotic and mitotic modes of spindle assembly.

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