Protection Against the Allergic Airway Inflammation Depends on the Modulation of Spleen Dendritic Cell Function and Induction of Regulatory T Cells in Mice

Overview

Journal Genet Vaccines Ther

Publisher Biomed Central

Date 2010 Mar 26

PMID 20334668

Citations 1

Authors

Yaoli Wang

Chunxue Bai

Guansong Wang

Diane Wang

Xiaoming Cheng

Jian Huang

Dongpo Jiang

Guisheng Qian

Xiangdong Wang

Affiliations

Soon will be listed here.

Abstract

Background: Allergen-induced imbalance of specific T regulatory (Treg) cells and T helper 2 cells plays a decisive role in the development of immune response against allergens.

Objective: To evaluate effects and potential mechanisms of DNA vaccine containing ovalbumin (OVA) and Fc fusion on allergic airway inflammation.

Methods: Bronchoalveolar lavage (BAL) levels of inflammatory mediators and leukocyte infiltration, expression of CD11c+CD80+ and CD11c+CD86+ co-stimulatory molecules in spleen dendritic cells (DCs), circulating CD4+ and CD8+ T cells, Foxp3+ in spleen CD4+ T cells and spleen CD4+ T cells were measured in OVA-sensitized and challenged animals pretreated with pcDNA, OVA-pcDNA, Fc-pcDNA, and OVA-Fc-pcDNA.

Results: OVA-Sensitized and challenged mice developed airway inflammation and Th2 responses, and decreased the proliferation of peripheral CD4+and CD8+ T cells and the number of spleen Foxp3+ Treg. Those changes with increased INF-gamma production and reduced OVA-specific IgE production were protected by the pretreatment with OVA-Fc-pcDNA.

Conclusion: DNA vaccine encoding both Fc and OVA showed more effective than DNA vaccine encoding Fc or OVA alone, through the balance of DCs and Treg.

Citing Articles

Airway delivery of soluble factors from plastic-adherent bone marrow cells prevents murine asthma.

Ionescu L, Alphonse R, Arizmendi N, Morgan B, Abel M, Eaton F Am J Respir Cell Mol Biol. 2011; 46(2):207-16.

PMID: 21903873 PMC: 3361359. DOI: 10.1165/rcmb.2010-0391OC.

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