GanedenBC30 Cell Wall and Metabolites: Anti-inflammatory and Immune Modulating Effects in Vitro

Overview

Journal BMC Immunol

Publisher Biomed Central

Specialty Allergy & Immunology

Date 2010 Mar 25

PMID 20331905

Citations 46

Authors

Gitte S Jensen

Kathleen F Benson

Steve G Carter

John R Endres

Affiliations

Soon will be listed here.

Abstract

Background: This study was performed to evaluate anti-inflammatory and immune modulating properties of the probiotic, spore-forming bacterial strain: Bacillus coagulans: GBI-30, (PTA-6086, GanedenBC30TM). In addition, cell wall and metabolite fractions were assayed separately to address whether biological effects were due to cell wall components only, or whether secreted compounds from live bacteria had additional biological properties. The spores were heat-activated, and bacterial cultures were grown. The culture supernatant was harvested as a source of metabolites (MTB), and the bacteria were used to isolate cell wall fragments (CW). Both of these fractions were compared in a series of in vitro assays.

Results: Both MTB and CW inhibited spontaneous and oxidative stress-induced ROS formation in human PMN cells and increased the phagocytic activity of PMN cells in response to bacteria-like carboxylated fluorospheres. Both fractions supported random PMN and f-MLP-directed PMN cell migration, indicating a support of immune surveillance and antibacterial defense mechanisms. In contrast, low doses of both fractions inhibited PMN cell migration towards the inflammatory mediators IL-8 and LTB4. The anti-inflammatory activity was strongest for CW, where the PMN migration towards IL-8 was inhibited down to dilutions of 1010.Both MTB and CW induced the expression of the CD69 activation marker on human CD3- CD56+ NK cells, and enhanced the expression of CD107a when exposed to K562 tumor cells in vitro.The fractions directly modulated cytokine production, inducing production of the Th2 cytokines IL-4, IL-6, and IL-10, and inhibiting production of IL-2.Both fractions further modulated mitogen-induced cytokine production in the following manner: Both fractions enhanced the PHA-induced production of IL-6 and reduced the PHA-induced production of TNF-alpha. Both fractions enhanced the PWM-induced production of TNF-alpha and IFN-gamma. In addition, MTB also enhanced both the PHA- and the PWM-induced expression of IL-10.

Conclusion: The data suggest that consumption of GanedenBC30TM may introduce both cell wall components and metabolites that modulate inflammatory processes in the gut. Both the cell wall and the supernatant possess strong immune modulating properties in vitro. The anti-inflammatory effects, combined with direct induction of IL-10, are of interest with respect to possible treatment of inflammatory bowel diseases as well as in support of a healthy immune system.

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References

Harris G, KuoLee R, Chen W . Role of Toll-like receptors in health and diseases of gastrointestinal tract. World J Gastroenterol. 2006; 12(14):2149-60. PMC: 4087639. DOI: 10.3748/wjg.v12.i14.2149. View

Hun L . Bacillus coagulans significantly improved abdominal pain and bloating in patients with IBS. Postgrad Med. 2009; 121(2):119-24. DOI: 10.3810/pgm.2009.03.1984. View

Casula G, Cutting S . Bacillus probiotics: spore germination in the gastrointestinal tract. Appl Environ Microbiol. 2002; 68(5):2344-52. PMC: 127533. DOI: 10.1128/AEM.68.5.2344-2352.2002. View

Moretto M, Weiss L, Combe C, Khan I . IFN-gamma-producing dendritic cells are important for priming of gut intraepithelial lymphocyte response against intracellular parasitic infection. J Immunol. 2007; 179(4):2485-92. PMC: 3109618. DOI: 10.4049/jimmunol.179.4.2485. View

Honzel D, Carter S, Redman K, Schauss A, Endres J, Jensen G . Comparison of chemical and cell-based antioxidant methods for evaluation of foods and natural products: generating multifaceted data by parallel testing using erythrocytes and polymorphonuclear cells. J Agric Food Chem. 2008; 56(18):8319-25. DOI: 10.1021/jf800401d. View

Bezkorovainy A . Probiotics: determinants of survival and growth in the gut. Am J Clin Nutr. 2001; 73(2 Suppl):399S-405S. DOI: 10.1093/ajcn/73.2.399s. View

Neurath M, Finotto S, Glimcher L . The role of Th1/Th2 polarization in mucosal immunity. Nat Med. 2002; 8(6):567-73. DOI: 10.1038/nm0602-567. View

Jensen G, Hart A, Schauss A . An antiinflammatory immunogen from yeast culture induces activation and alters chemokine receptor expression on human natural killer cells and B lymphocytes in vitro. Nutr Res. 2020; 27(6):327-335. DOI: 10.1016/j.nutres.2007.04.008. View

Alter G, Malenfant J, Altfeld M . CD107a as a functional marker for the identification of natural killer cell activity. J Immunol Methods. 2004; 294(1-2):15-22. DOI: 10.1016/j.jim.2004.08.008. View

10.

Draing C, Sigel S, Deininger S, Traub S, Munke R, Mayer C . Cytokine induction by Gram-positive bacteria. Immunobiology. 2008; 213(3-4):285-96. DOI: 10.1016/j.imbio.2007.12.001. View

11.

Hart A, Zaske L, Patterson K, Drapeau C, Jensen G . Natural killer cell activation and modulation of chemokine receptor profile in vitro by an extract from the cyanophyta Aphanizomenon flos-aquae. J Med Food. 2007; 10(3):435-41. DOI: 10.1089/jmf.2007.401. View

12.

Dominguez-Bello M, Blaser M . Do you have a probiotic in your future?. Microbes Infect. 2008; 10(9):1072-6. PMC: 2613679. DOI: 10.1016/j.micinf.2008.07.036. View

13.

Li W, Brackett B, Halper J . Culture supernatant of Lactobacillus acidophilus stimulates proliferation of embryonic cells. Exp Biol Med (Maywood). 2005; 230(7):494-500. DOI: 10.1177/153537020523000708. View

14.

Hormannsperger G, Haller D . Molecular crosstalk of probiotic bacteria with the intestinal immune system: clinical relevance in the context of inflammatory bowel disease. Int J Med Microbiol. 2009; 300(1):63-73. DOI: 10.1016/j.ijmm.2009.08.006. View

15.

Turnbaugh P, Ley R, Mahowald M, Magrini V, Mardis E, Gordon J . An obesity-associated gut microbiome with increased capacity for energy harvest. Nature. 2006; 444(7122):1027-31. DOI: 10.1038/nature05414. View

16.

Graff S, Chaumeil J, Boy P, Lai-Kuen R, Charrueau C . Formulations for protecting the probiotic Saccharomyces boulardii from degradation in acidic condition. Biol Pharm Bull. 2008; 31(2):266-72. DOI: 10.1248/bpb.31.266. View

17.

Rescigno M, Lopatin U, Chieppa M . Interactions among dendritic cells, macrophages, and epithelial cells in the gut: implications for immune tolerance. Curr Opin Immunol. 2008; 20(6):669-75. DOI: 10.1016/j.coi.2008.09.007. View

18.

Duerkop B, Vaishnava S, Hooper L . Immune responses to the microbiota at the intestinal mucosal surface. Immunity. 2009; 31(3):368-76. DOI: 10.1016/j.immuni.2009.08.009. View

19.

Ostad S, Salarian A, Ghahramani M, Fazeli M, Samadi N, Jamalifar H . Live and heat-inactivated lactobacilli from feces inhibit Salmonella typhi and Escherichia coli adherence to Caco-2 cells. Folia Microbiol (Praha). 2009; 54(2):157-60. DOI: 10.1007/s12223-009-0024-7. View

20.

de Vrese M, Schrezenmeir J . Probiotics, prebiotics, and synbiotics. Adv Biochem Eng Biotechnol. 2008; 111:1-66. DOI: 10.1007/10_2008_097. View