A Preliminary Immunologic Study of Urinary Proteins: the Questionable Value of Protein Clearances in Kidney Disease

Overview

Journal Can Med Assoc J

Specialty General Medicine

Date 2010 Mar 24

PMID 20328484

Citations 1

Authors

R Barcelo

V E POLLAK

Affiliations

Soon will be listed here.

Abstract

The clearances of seven different proteins were measured by a quantitative immunodiffusion technique in 15 patients with proteinuria. All urines were also studied by immunoelectrophoresis.The renal histology was evaluated in each case, and no correlation was found between histologic changes and the urinary protein excretion. This observation was confirmed by both immunodiffusion and immunoelectrophoretic techniques. No specific urinary protein excretion pattern was found in six patients with systemic lupus erythematosus.High-molecular-weight proteins were rarely found in urine, even when the glomerular basement membrane was definitely thickened. Low-molecular-weight proteins were often observed, but their clearances were variable. The results do not support the suggestion that protein clearances are valuable diagnostic and prognostic tools in renal diseases. They also do not support the view that glomerular filtration is the sole factor responsible for the final patterns of urinary proteins; tubular reabsorption is probably another important factor.

Citing Articles

Clinical and morphological aspects of nephrotic syndrome in perimembranous, focally sclerosing and membrano-proliferative glomerulonephritis.

Milewski C, Konig M, von Gise H, Bohle A Klin Wochenschr. 1983; 61(10):493-7.

PMID: 6876682 DOI: 10.1007/BF01488716.

References

Takahashi S, Schmid K . A low-molecular-weight gamma1-globulin derived from normal human plasma. Biochim Biophys Acta. 1962; 63:343. DOI: 10.1016/0006-3002(62)90690-x. View

JOACHIM G, Cameron J, Schwartz M, Becker E . SELECTIVITY OF PROTEIN EXCRETION IN PATIENTS WITH THE NEPHROTIC SYNDROME. J Clin Invest. 1964; 43:2332-46. PMC: 289661. DOI: 10.1172/JCI105107. View

MUEHRCKE R, KARK R, PIRANI C . Technique of percutaneous renal biopsy in the prone position. J Urol. 1955; 74(3):267-77. DOI: 10.1016/S0022-5347(17)67279-3. View

Hartmann L, LAGRUE G, MORETTI J . [Comparison of the nephrotic syndrome & orthostatic proteinuria; physical & immunological studies of blood & urinary proteins]. Rev Fr Etud Clin Biol. 1958; 3(10):1052-61. View

Davies J . Cytological evidence of protein absorption in fetal and adult mammalian kidneys. Am J Anat. 1954; 94(1):45-71. DOI: 10.1002/aja.1000940103. View

Straus W . CYTOCHEMICAL OBSERVATIONS ON THE RELATIONSHIP BETWEEN LYSOSOMES AND PHAGOSOMES IN KIDNEY AND LIVER BY COMBINED STAINING FOR ACID PHOSPHATASE AND INTRAVENOUSLY INJECTED HORSERADISH PEROXIDASE. J Cell Biol. 1964; 20:497-507. PMC: 2106412. DOI: 10.1083/jcb.20.3.497. View

Cameron J, White R . SELECTIVITY OF PROTEINURIA IN CHILDREN WITH THE NEPHROTIC SYNDROME. Lancet. 1965; 1(7383):463-5. DOI: 10.1016/s0140-6736(65)91593-x. View

Barcelo R, RIOPEL P, LEGRESLEY L . [Immunoelectrophoresis: its technic, interpretation and applications]. Union Med Can. 1961; 90:1235-45. View

Rowe D, Soothill J . Serum proteins in normal urine. Clin Sci. 1961; 21:75-85. View

10.

Miller F, Palade G . LYTIC ACTIVITIES IN RENAL PROTEIN ABSORPTION DROPLETS. AN ELECTRON MICROSCOPICAL CYTOCHEMICAL STUDY. J Cell Biol. 1964; 23:519-52. PMC: 2106540. DOI: 10.1083/jcb.23.3.519. View

11.

Soothill J . Estimation of eight serum proteins by a gel diffusion precipitin technique. J Lab Clin Med. 1962; 59:859-70. View

12.

Oliver J, Macdowell M, Lee Y . Cellular mechanisms of protein metabolism in the nephron. I. The structural aspects of proteinuria; tubular absorption, droplet formation, and the disposal of proteins. J Exp Med. 1954; 99(6):589-604. PMC: 2136352. DOI: 10.1084/jem.99.6.589. View

13.

Takatsuki K, Osserman E . DEMONSTRATION OF TWO TYPES OF LOW MOLECULAR WEIGHT GAMMA-GLOBULINS IN NORMAL HUMAN URINE. J Immunol. 1964; 92:100-7. View

14.

Hardwicke J, SQUIRE J . The relationship between plasma albumin concentration and protein excretion in patients with proteinuria. Clin Sci. 1955; 14(3):509-30. View

15.

PATTE J, BALDASSAIRE G, LORET J . [Immuno-electrophoretic study of normal and pathological proteinurias]. Rev Fr Etud Clin Biol. 1958; 3(9):960-70. View

16.

McKay E, Slater R . STUDIES ON HUMAN PROTEINURIA. II. SOME CHARACTERISTICS OF THE GAMMA GLOBULINS EXCRETED IN NORMAL, EXERCISE, POSTURAL, AND NEPHROTIC PROTEINURIA. J Clin Invest. 1962; 41(8):1638-52. PMC: 291081. DOI: 10.1172/JCI104621. View