Characterization of 13 Multi-drug Resistant Salmonella Serovars from Different Broiler Chickens Associated with Those of Human Isolates

Overview

Journal BMC Microbiol

Publisher Biomed Central

Specialty Microbiology

Date 2010 Mar 24

PMID 20307324

Citations 12

Authors

Lan-Ho Chiu

Cheng-Hsun Chiu

Yan-Ming Horn

Chien-Shun Chiou

Chien-Yu Lee

Chia-Ming Yeh

Chang-You Yu

Chean-Ping Wu

Chao-Chin Chang

Chishih Chu

Affiliations

Soon will be listed here.

Abstract

Background: Salmonella are frequently isolated from chickens and their products. Prevalent serogroups and serovars of Salmonella as well as their genotypes and antibiograms were determined for cloacal samples from 1595 chickens. To understand the possible serovar and H antigens for transmission between chicken and human, serovars and their H antigens of 164 chicken and 5314 human isolates were compared.

Results: Prevalence of Salmonella differed among chicken lines and ages. Chicken and human isolates belonged mainly to serogroup B, C1, C2-C3, D, and E. 13 serovars and 66 serovars were identified for chicken and human isolates respectively. The common serovars for chicken and human isolates were S. Typhimurium, S. Enteritidis, S. Albany, S. Derby, and S. Anatum and shared common H1 antigens "g complex; i; e,h; and z4,z24" and H2 antigens "1 complex and -". In human isolates, H1 antigen "i" and H2 antigen "-" were common in all serogroups. In chicken, antimicrobial susceptibility differed among serogroups, serovars and three counties. All isolates were susceptible to cefazolin and ceftriaxone, but highly resistant to ampicillin, chloramphenicol, flumequine, streptomycin, sulfamethoxazole-trimethoprim, and tetracycline. Except those isolates of serogroup C1 of Chick group and serogroup G, all isolates were multi-drug resistance. Only S. Kubacha, S. Typhimurium, S. Grampian, and S. Mons were resistant to ciprofloxacin and/or enrofloxacin.

Conclusion: In chicken, prevalent serogroups and serovars were associated with chicken ages, lines and regions; and flouroquinolone-resistant and MDR isolates emerged. H1 antigens "g complex and i" and H2 antigens "1 complex and -" might be important for transmission of Salmonella between chicken and human.

Citing Articles

Ginsenoside Ro improves Salmonella Typhimurium-induced colitis through inhibition of the virulence factors SopB and SopE2 via the RAC1/CDC42/ARP2/3 pathway.

Cui X, Wang Y, Liu Z, Zhao M, Zhu M, Yu W FASEB J. 2024; 38(24):e70282.

PMID: 39734277 PMC: 11695707. DOI: 10.1096/fj.202401712R.

Salmonella enteritidis acquires phage resistance through a point mutation in rfbD but loses some of its environmental adaptability.

Zeng Y, Li P, Liu S, Shen M, Liu Y, Zhou X Vet Res. 2024; 55(1):85.

PMID: 38970094 PMC: 11227202. DOI: 10.1186/s13567-024-01341-7.

Metabolomic and microbiome analysis of the protective effects of Puerarin against Salmonella Enteritidis Infection in chicks.

Lu Y, Ge S, Zhang H, Lu W, Bao X, Pan S BMC Vet Res. 2023; 19(1):242.

PMID: 37990191 PMC: 10664526. DOI: 10.1186/s12917-023-03806-x.

Antimicrobial resistance profiling and burden of resistance genes in zoonotic Salmonella isolated from broiler chicken.

Das T, Rana E, Dutta A, Bostami M, Rahman M, Deb P Vet Med Sci. 2021; 8(1):237-244.

PMID: 34599790 PMC: 8788989. DOI: 10.1002/vms3.648.

The Occurrence of Antimicrobial-Resistant in Hatcheries and Dissemination in an Integrated Broiler Chicken Operation in Korea.

Shang K, Wei B, Cha S, Zhang J, Park J, Lee Y Animals (Basel). 2021; 11(1).

PMID: 33440890 PMC: 7827806. DOI: 10.3390/ani11010154.

References

Gast R, Guraya R, Guard-Bouldin J, Holt P . In vitro penetration of egg yolks by Salmonella Enteritidis and Salmonella Heidelberg strains during thirty-six-hour ambient temperature storage. Poult Sci. 2007; 86(7):1431-5. DOI: 10.1093/ps/86.7.1431. View

Wong H, Odoms K, Pyles R . Salmonella flagellin-dependent proinflammatory responses are localized to the conserved amino and carboxyl regions of the protein. J Immunol. 2001; 167(12):7009-16. DOI: 10.4049/jimmunol.167.12.7009. View

de la Torre E, Zapata D, Tello M, Mejia W, Frias N, Garcia Pena F . Several Salmonella enterica subsp. enterica serotype 4,5,12:i:- phage types isolated from swine samples originate from serotype typhimurium DT U302. J Clin Microbiol. 2003; 41(6):2395-400. PMC: 156524. DOI: 10.1128/JCM.41.6.2395-2400.2003. View

Andersen-Nissen E, Smith K, Strobe K, Barrett S, Cookson B, Logan S . Evasion of Toll-like receptor 5 by flagellated bacteria. Proc Natl Acad Sci U S A. 2005; 102(26):9247-52. PMC: 1166605. DOI: 10.1073/pnas.0502040102. View

Mortimer C, Peters T, Gharbia S, Logan J, Arnold C . Towards the development of a DNA-sequence based approach to serotyping of Salmonella enterica. BMC Microbiol. 2004; 4:31. PMC: 514894. DOI: 10.1186/1471-2180-4-31. View

Rigby C, Pettit J, Baker M, BENTLEY A, Salomons M, Lior H . Flock infection and transport as sources of salmonellae in broiler chickens and carcasses. Can J Comp Med. 1980; 44(3):328-37. PMC: 1320082. View

Murase T, Senjyu K, Maeda T, Tanaka M, Sakae H, Matsumoto Y . Monitoring of chicken houses and an attached egg-processing facility in a laying farm for Salmonella contamination between 1994 and 1998. J Food Prot. 2002; 64(12):1912-6. DOI: 10.4315/0362-028x-64.12.1912. View

Chiu C, Su L, Chu C, Wang M, Yeh C, Weill F . Detection of multidrug-resistant Salmonella enterica serovar typhimurium phage types DT102, DT104, and U302 by multiplex PCR. J Clin Microbiol. 2006; 44(7):2354-8. PMC: 1489530. DOI: 10.1128/JCM.00171-06. View

Vaeteewootacharn K, Sutra S, Vaeteewootacharn S, Sithigon D, Jamjane O, Chomvarin C . Salmonellosis and the food chain in Khon Kaen, northeastern Thailand. Southeast Asian J Trop Med Public Health. 2005; 36(1):123-9. View

10.

Kado C, Liu S . Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981; 145(3):1365-73. PMC: 217141. DOI: 10.1128/jb.145.3.1365-1373.1981. View

11.

Tallant T, Deb A, Kar N, Lupica J, de Veer M, DiDonato J . Flagellin acting via TLR5 is the major activator of key signaling pathways leading to NF-kappa B and proinflammatory gene program activation in intestinal epithelial cells. BMC Microbiol. 2004; 4:33. PMC: 516440. DOI: 10.1186/1471-2180-4-33. View

12.

Berghold C, Kornschober C, Lederer I, Allerberger F . Occurrence of Salmonella Enteritidis phage type 29 in Austria: an opportunity to assess the relevance of chicken meat as source of human Salmonella infections. Euro Surveill. 2004; 9(10):31-4. View

13.

van Duijkeren E, Wannet W, Houwers D, Van Pelt W . Serotype and phage type distribution of salmonella strains isolated from humans, cattle, pigs, and chickens in the Netherlands from 1984 to 2001. J Clin Microbiol. 2002; 40(11):3980-5. PMC: 139702. DOI: 10.1128/JCM.40.11.3980-3985.2002. View

14.

Snow L, Davies R, Christiansen K, Carrique-Mas J, Wales A, OConnor J . Survey of the prevalence of Salmonella species on commercial laying farms in the United Kingdom. Vet Rec. 2007; 161(14):471-6. DOI: 10.1136/vr.161.14.471. View

15.

Yoshida C, Franklin K, Konczy P, McQuiston J, Fields P, Nash J . Methodologies towards the development of an oligonucleotide microarray for determination of Salmonella serotypes. J Microbiol Methods. 2007; 70(2):261-71. DOI: 10.1016/j.mimet.2007.04.018. View

16.

Angkititrakul S, Chomvarin C, Chaita T, Kanistanon K, Waethewutajarn S . Epidemiology of antimicrobial resistance in Salmonella isolated from pork, chicken meat and humans in Thailand. Southeast Asian J Trop Med Public Health. 2006; 36(6):1510-5. View

17.

Chang C, Lin Y, Chang C, Yeh K, Chiu C, Chu C . Epidemiologic relationship between fluoroquinolone-resistant Salmonella enterica Serovar Choleraesuis strains isolated from humans and pigs in Taiwan (1997 to 2002). J Clin Microbiol. 2005; 43(6):2798-804. PMC: 1151913. DOI: 10.1128/JCM.43.6.2798-2804.2005. View

18.

Gaul S, Wedel S, Erdman M, Harris D, Harris I, Ferris K . Use of pulsed-field gel electrophoresis of conserved XbaI fragments for identification of swine Salmonella serotypes. J Clin Microbiol. 2006; 45(2):472-6. PMC: 1829035. DOI: 10.1128/JCM.00962-06. View

19.

Levings R, Djordjevic S, Hall R . SGI2, a relative of Salmonella genomic island SGI1 with an independent origin. Antimicrob Agents Chemother. 2008; 52(7):2529-37. PMC: 2443934. DOI: 10.1128/AAC.00189-08. View

20.

Pieskus J, Milius J, Michalskiene I, Zagrebneviene G . The distribution of Salmonella serovars in chicken and humans in Lithuania. J Vet Med A Physiol Pathol Clin Med. 2006; 53(1):12-6. DOI: 10.1111/j.1439-0442.2006.00789.x. View